Fatma A Eissa1, Hani Choudhry1,2,3, Wesam H Abdulaal1,3, Othman A Baothman1, Mustafa Zeyadi1, Said S Moselhy1,4,5,6, Mazin A Zamzami1,2,3. 1. Biochemistry Department, Faculty of science, King Abdulaziz University. 2. Cancer and mutagensis Unit, King Fahad Medical Research Center, KingAbdulaziz University. 3. Cancer Metabolism and Epigenetic Unit, Faculty of Science, King Abdulaziz University. 4. Experimental biochemistry unit, King Fahad Medical Research center (KFMRC), King Abdulaziz University P.O. Box 21424, Jeddah, Saudi Arabia. 5. Bioactive Natural Products Research Group, King Abdulaziz University. 6. Department of Biochemistry, Faculty of Science, Ain Shams University.
Abstract
BACKGROUND: Hypercholesterolemia is a major risk factor for development of atherosclerosis. The present study was conducted to evaluate the potential effect of ginger oil alone or combined with rosemary oil as hypocholesterolemic agent in rats fed high fat diet. MATERIALS AND METHODS: Healthy female albino rats (n=80) weighting about (150-180 g) were included in this study divided into two equal groups; Group (I): were fed on the basal diet. Group (I) were divided into 4 subgroups each 10: Group (Ia): negative control. Group (Ib): Rats received i.p 2.5 g/Kg b.w of ginger oil. Group (Ic): rats received i.p 2.5 g/Kg b.w of rosemary oil. Group (Id): Rats received i.p 5 g/Kg b.w mixture of ginger oil and rosemary oil (1:1). The second main groups; Group (II): high fat diet (HFD) were fed on the basal diet plus cholesterol (1%), bile salt (0.25%) and animal fat (15%) to induce hypercholesterolemia for six weeks. Group (II) was divided into 4subgroups: Group (IIa): HFD. Group (IIb): HFD were treated with i.p 2.5 g/Kg b.w ginger oil. Group (IIc): (n=10) HFD were treated with i.p 2.5 g/Kg b.w rosemary oil. Group (IId): (n=10) HFD were treated with i.p 5 g/Kg b.w mixture of oils. RESULTS: It was found that HFD rats showed a significant elevation in glucose, total cholesterol, triglyceride, GOT, GPT, alkaline phosphatase and a reduction in serum HDL-c compared with negative control. Treatment with ginger oil, rosemary oil and their mixture modulated the elevation of these parameters. Histopathological examination of the liver tissue of HFD rats showed a lipid deposition and macrophage infiltration and stenosis of hepatic vein. Treatment with mixture oils preserves normal structure of liver. CONCLUSION: It was concluded that, hypocholesterolemic effect was related to the active oil content as Rosemary oil contain - α-pinene, Camphor, cineole, borneol and Ginger oil contain Linalool, Terpineol, Borneol, Eucalyptol.
BACKGROUND:Hypercholesterolemia is a major risk factor for development of atherosclerosis. The present study was conducted to evaluate the potential effect of gingeroil alone or combined with rosemaryoil as hypocholesterolemic agent in rats fed high fat diet. MATERIALS AND METHODS: Healthy female albino rats (n=80) weighting about (150-180 g) were included in this study divided into two equal groups; Group (I): were fed on the basal diet. Group (I) were divided into 4 subgroups each 10: Group (Ia): negative control. Group (Ib): Rats received i.p 2.5 g/Kg b.w of gingeroil. Group (Ic): rats received i.p 2.5 g/Kg b.w of rosemaryoil. Group (Id): Rats received i.p 5 g/Kg b.w mixture of gingeroil and rosemaryoil (1:1). The second main groups; Group (II): high fat diet (HFD) were fed on the basal diet plus cholesterol (1%), bile salt (0.25%) and animal fat (15%) to induce hypercholesterolemia for six weeks. Group (II) was divided into 4subgroups: Group (IIa): HFD. Group (IIb): HFD were treated with i.p 2.5 g/Kg b.w gingeroil. Group (IIc): (n=10) HFD were treated with i.p 2.5 g/Kg b.w rosemaryoil. Group (IId): (n=10) HFD were treated with i.p 5 g/Kg b.w mixture of oils. RESULTS: It was found that HFD rats showed a significant elevation in glucose, total cholesterol, triglyceride, GOT, GPT, alkaline phosphatase and a reduction in serum HDL-c compared with negative control. Treatment with gingeroil, rosemaryoil and their mixture modulated the elevation of these parameters. Histopathological examination of the liver tissue of HFD rats showed a lipid deposition and macrophage infiltration and stenosis of hepatic vein. Treatment with mixtureoils preserves normal structure of liver. CONCLUSION: It was concluded that, hypocholesterolemic effect was related to the active oil content as Rosemaryoil contain - α-pinene, Camphor, cineole, borneol and Gingeroil contain Linalool, Terpineol, Borneol, Eucalyptol.
Authors: Paul M Ridker; Jacques Genest; S Matthijs Boekholdt; Peter Libby; Antonio M Gotto; Børge G Nordestgaard; Samia Mora; Jean G MacFadyen; Robert J Glynn; John J P Kastelein Journal: Lancet Date: 2010-07-23 Impact factor: 79.321