| Literature DB >> 28624244 |
Yuichi Kato1, Shih-Hsin Ho2, Christopher J Vavricka1, Jo-Shu Chang3, Tomohisa Hasunuma4, Akihiko Kondo5.
Abstract
The aim of this study was to improve biomass production of the green microalga Chlamydomonas sp. JSC4 under high salinity conditions. For this purpose, heavy ion beam-coupled mutagenesis and evolutionary engineering were performed using JSC4 as the parent strain. After long-term and continuous cultivation with high salinity, salt-resistant strains that grow well even in the presence of 7% sea salt were successfully obtained. Transcriptional analysis revealed inactivation of starch-to-lipid biosynthesis switching, which resulted in delayed starch degradation and decreased lipid content in the salt-resistant strains. Cellular aggregation and hypertrophy during high salinity were relieved in these strains, indicating strong resistance to salt stress. These results suggest that high salinity stress, not the salinity condition itself, is important for activating lipid accumulation mechanisms in microalgae.Entities:
Keywords: Biofuels; Evolutionary breeding; Microalgae; Salinity stress
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Year: 2017 PMID： 28624244 DOI： 10.1016/j.biortech.2017.06.035
Source DB: PubMed Journal: Bioresour Technol ISSN： 0960-8524 Impact factor: 9.642