Wadha Alfouzan1, Tahani Al-Enezi2, Ebteehal AlRoomi3, Vayalil Sandhya3, Rachel Chandy4, Zia Uddin Khan4. 1. Microbiology Unit, Department of Laboratories, Farwania Hospital, Kuwait; Department of Microbiology, Faculty of Medicine, Health Sciences Center, Kuwait University, Kuwait. Electronic address: alfouzan.w@hsc.edu.kw. 2. Microbiology Unit, Department of Laboratories, Farwania Hospital, Kuwait. 3. Microbiology Unit, Department of Laboratories, Mubarak Alkabeer Hospital, Kuwait. 4. Department of Microbiology, Faculty of Medicine, Health Sciences Center, Kuwait University, Kuwait.
Abstract
BACKGROUND: Candida species are part of the normal human microbiota. However, in recent years, nosocomial bloodstream Candida infections have emerged as a significant problem ranking the fourth common cause of fungemia in intensive care units. Although microdilution methods are the ones recommended for susceptibility testing, they are difficult to undertake in the clinical practice. Thus, an automated commercially available test is ideal. AIMS: To compare minimum inhibitory concentrations (MICs) obtained with the recently introduced Vitek 2 yeast susceptibility system card (AST-YS01) with Etest. METHODS: 263 clinical Candida isolates representing six species were included in the study. Categorical agreements (CA) were assessed as described elsewhere. RESULTS: Irrespective of the Candida species tested, the overall CA between Vitek 2 and Etest ranged between 66.7% and 100%. In general, Etest yielded lower MICs than Vitek 2. For Candida albicans, the CA between Vitek 2 and Etest was >95% for amphotericin B, voriconazole and flucytosine, but only 89% for fluconazole. With respect to Candida glabrata, the CA was between 97% and 100%. The major errors were with Candida krusei and flucytosine and Candida kefyr and amphotericin B. Candida tropicalis susceptibility for fluconazole by Vitek 2 reported more SDD and resistant strains than Etest. Candida parapsilosis showed 100% CA against all the four antifungals tested. No very major errors were detected between the two methods. CONCLUSIONS: Vitek 2 provided comparable results to Etest with quick turnaround for the testing of Candida species susceptibilities.
BACKGROUND:Candida species are part of the normal human microbiota. However, in recent years, nosocomial bloodstream Candida infections have emerged as a significant problem ranking the fourth common cause of fungemia in intensive care units. Although microdilution methods are the ones recommended for susceptibility testing, they are difficult to undertake in the clinical practice. Thus, an automated commercially available test is ideal. AIMS: To compare minimum inhibitory concentrations (MICs) obtained with the recently introduced Vitek 2 yeast susceptibility system card (AST-YS01) with Etest. METHODS: 263 clinical Candida isolates representing six species were included in the study. Categorical agreements (CA) were assessed as described elsewhere. RESULTS: Irrespective of the Candida species tested, the overall CA between Vitek 2 and Etest ranged between 66.7% and 100%. In general, Etest yielded lower MICs than Vitek 2. For Candida albicans, the CA between Vitek 2 and Etest was >95% for amphotericin B, voriconazole and flucytosine, but only 89% for fluconazole. With respect to Candida glabrata, the CA was between 97% and 100%. The major errors were with Candida krusei and flucytosine and Candida kefyr and amphotericin B. Candidatropicalis susceptibility for fluconazole by Vitek 2 reported more SDD and resistant strains than Etest. Candida parapsilosis showed 100% CA against all the four antifungals tested. No very major errors were detected between the two methods. CONCLUSIONS: Vitek 2 provided comparable results to Etest with quick turnaround for the testing of Candida species susceptibilities.
Authors: G Jiménez-Guerra; I Casanovas Moreno-Torres; M Gutiérrez-Soto; F Vazquez-Alonso; A Sorlózano-Puerto; J M Navarro-Marí; J Gutiérrez-Fernández Journal: Rev Esp Quimioter Date: 2018-06-20 Impact factor: 1.553
Authors: Antonio Sorlozano-Puerto; Maria Albertuz-Crespo; Isaac Lopez-Machado; Lidia Gil-Martinez; Juan Jose Ariza-Romero; Alba Maroto-Tello; Alberto Baños-Arjona; Jose Gutierrez-Fernandez Journal: Pharmaceuticals (Basel) Date: 2020-12-29