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Literature DB >> 28616445

Nitroxide Labeling of Proteins and the Determination of Paramagnetic Relaxation Derived Distance Restraints for NMR Studies.

Abstract

Site-specific attachment of paramagnetic spin labels to biomolecules causes distance-dependent line-broadening effects, which can be exploited to study the structure and dynamics of these molecules in solution. This protocol describes how to attach nitroxide spin labels to proteins and how to collect and analyze NMR data using these labeled samples. We also explain how to derive distance restraints for paramagnetic relaxation enhancement nuclear magnetic resonance (PRE-NMR) studies.

Entities: Chemical

Keywords: Distance restraints; Large protein NMR; Nitroxide spin label; Nuclear magnetic resonance spectroscopy; Paramagnetic relaxation enhancement

Year: 2017 PMID： 28616445 PMCID： PMC5467883 DOI： 10.21769/BioProtoc.2207

Source DB: PubMed Journal: Bio Protoc ISSN： 2331-8325

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