Literature DB >> 28611505

Development and Optimization of a Reverse Transcription Hemi-Nested PCR Primer for the Detection of Potato Mop-Top Virus at Quarantine Inspection Sites in Korea.

S Lee1, J Y Lee2, H Kim3, J H Kim2, J Y Rho2.   

Abstract

Potato mop-top virus (PMTV) is classified as a plant quarantine virus in Korea. It is tested at import inspection sites. In this study, two sets of reverse transcription-hemi-nested polymerase chain reaction primers were developed to diagnose PMTV. In addition, modified- positive control plasmid was developed for the identification of false positive results in plant quarantine. The assay is expected to be useful for improving the detection and diagnosis of PMTV.

Entities:  

Keywords:  Hemi-nested polymerase chain reaction; PMTV; Potato mop-top virus; Quarantine

Year:  2016        PMID： 28611505      PMCID： PMC5446823          DOI： 10.1007/s12088-016-0623-3

Source DB:  PubMed          Journal:  Indian J Microbiol        ISSN： 0046-8991            Impact factor:   2.461


  7 in total

1.  Use of DNA methyltransferase/endonuclease enzyme combinations for megabase mapping of chromosomes.

Authors:  M Nelson; M McClelland
Journal:  Methods Enzymol       Date:  1992       Impact factor: 1.600

2.  Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses.

Authors:  M F Clark; A N Adams
Journal:  J Gen Virol       Date:  1977-03       Impact factor: 3.891

3.  An assessment of the phylogenetic relationship among sugarcane and related taxa based on the nucleotide sequence of 5S rRNA intergenic spacers.

Authors:  Y B Pan; D M Burner; B L Legendre
Journal:  Genetica       Date:  2000       Impact factor: 1.082

4.  A new and sensitive Co-operational polymerase chain reaction for rapid detection of Ralstonia solanacearum in water.

Authors:  Paola Caruso; Edson Bertolini; Mariano Cambra; María M López
Journal:  J Microbiol Methods       Date:  2003-10       Impact factor: 2.363

5.  Detection of Potato mop top virus and Tobacco rattle virus Using a Multiplex Real-Time Fluorescent Reverse-Transcription Polymerase Chain Reaction Assay.

Authors:  R A Mumford; K Walsh; I Barker; N Boonham
Journal:  Phytopathology       Date:  2000-05       Impact factor: 4.025

6.  Detection of potato mop-top virus in soils and potato tubers using bait-plant bioassay, ELISA and RT-PCR.

Authors:  Muhammad Arif; Murad Ali; Anayatur Rehman; Muhammad Fahim
Journal:  J Virol Methods       Date:  2013-10-24       Impact factor: 2.014

7.  Development and Practical Use of RT-PCR for Seed-transmitted Prune dwarf virus in Quarantine.

Authors:  Siwon Lee; Yong-Gil Shin
Journal:  Plant Pathol J       Date:  2014-06       Impact factor: 1.795
  7 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.