| Literature DB >> 28606551 |
Yao Li1, Ya Yuan1, Lin Lei2, Fuhua Li1, Yueqiao Zhang1, Jinlong Chen1, Guohua Zhao2, Surui Wu3, Ran Yin4, Jian Ming5.
Abstract
The chemically carboxymethylated polysaccharide (CPMEP), which is derived from the purified polysaccharide from Morchella angusticepes Peck (PMEP), was prepared. The degree of substitution of CPMEP was 0.461. Fourier transform infrared spectra analysis and 13C, 1H, and DEPT135° NMR spectra indicated C6 position was partially substituted by CH2COOH. Moreover, serum total cholesterol of hypercholesterolemic rats treated with the high dose of PMEP and CPMEP was 1.54mmol/L and 1.29mmol/L compared with that of rats fed with a 2% cholesterol diet (1.74mmol/L), respectively, indicating CPMEP had a stronger cholesterol-lowering activity than PMEP. CPMEP had a trend in reducing liver total cholesterol while increasing the excretion of total fecal and intestinal bile acids, which were probably mediated by down-regulation of hepatic 3-hydroxy-3-methyl-glutaryl-coenzyme A reductase while up-regulation of cholesterol-7α-hydroxylase. It was therefore concluded that carboxymethylation of PMEP could enhance its cholesterol-lowering ability at least in rats.Entities:
Keywords: Carboxymethylation; Cholesterol metabolism; Morchella angusticepes Peck; Polysaccharide
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Year: 2017 PMID： 28606551 DOI： 10.1016/j.carbpol.2017.05.033
Source DB: PubMed Journal: Carbohydr Polym ISSN： 0144-8617 Impact factor: 9.381