Anoop K Enjeti1, Anita Ariyarajah2, Angel D'Crus2, Michael Seldon3, Lisa F Lincz4. 1. Haematology Department, Calvary Mater Newcastle, NSW, Australia; School of Medicine and Public Health, University of Newcastle, NSW, Australia; Pathology North-Hunter, New Lambton Heights, NSW, Australia; Hunter Medical Research Institute, New Lambton, Australia; Hunter Cancer Research Alliance, Waratah, NSW, Australia. Electronic address: Anoop.Enjeti@calvarymater.org.au. 2. Haematology Department, Calvary Mater Newcastle, NSW, Australia. 3. Haematology Department, Calvary Mater Newcastle, NSW, Australia; School of Medicine and Public Health, University of Newcastle, NSW, Australia; Pathology North-Hunter, New Lambton Heights, NSW, Australia. 4. Haematology Department, Calvary Mater Newcastle, NSW, Australia; Hunter Medical Research Institute, New Lambton, Australia; Hunter Cancer Research Alliance, Waratah, NSW, Australia; School of Biomedical Sciences and Pharmacy, University of Newcastle, NSW, Australia.
Abstract
BACKGROUND: Characterization of circulating microvesicles (MV) in healthy subjects in relation to various biological factors is not well studied. OBJECTIVES: We evaluated the influence of age, gender, smoking status, lipid and hormone profiles on circulating MV in healthy subjects. METHODS: Platelet free plasma from 143 volunteer blood donors (males=80, females=63) was evaluated by standardized flow cytometry for MV expressing CD41 (platelet-derived), CD105 (endothelial-derived), CD235 (red cell-derived), TF (tissue factor) and phosphatidylserine (PS) MV. Procoagulant function was measured by the Xa based assay (XaCT) and endogenous thrombin potential (ETP) using thrombin generation assay. RESULTS: Those ≤29years and ≥60years had higher levels of MV subsets (CD41, CD235, TF and PS) compared to those aged 30-59years. The median CD41, CD105, CD235, TF and PS expressing MV by flow cytometry were similar or lower in females, whilst procoagulant activity by the XaCT assay was higher (p=0.002). In smokers (n=21), certain MV subsets (CD41, TF and PS) and functional activity (ETP) was lower (p<0.05). Regression analysis showed that MV parameters of CD41, CD105, TF and ETP could be predicted independently by age, whilst smoking predicted for CD105, CD235, TF, PS and ETP. Certain MV parameters also correlated with BMI, lipid and hormone levels. The small RNA and miRNA levels did not differ by age group, smoking status or gender. CONCLUSIONS: It is important to recognize that differences may arise depending on age, gender, BMI, lipid, hormone levels and smoking status in apparently healthy subjects when evaluating MV for pathogenic potential.
BACKGROUND: Characterization of circulating microvesicles (MV) in healthy subjects in relation to various biological factors is not well studied. OBJECTIVES: We evaluated the influence of age, gender, smoking status, lipid and hormone profiles on circulating MV in healthy subjects. METHODS: Platelet free plasma from 143 volunteer blood donors (males=80, females=63) was evaluated by standardized flow cytometry for MV expressing CD41 (platelet-derived), CD105 (endothelial-derived), CD235 (red cell-derived), TF (tissue factor) and phosphatidylserine (PS) MV. Procoagulant function was measured by the Xa based assay (XaCT) and endogenous thrombin potential (ETP) using thrombin generation assay. RESULTS: Those ≤29years and ≥60years had higher levels of MV subsets (CD41, CD235, TF and PS) compared to those aged 30-59years. The median CD41, CD105, CD235, TF and PS expressing MV by flow cytometry were similar or lower in females, whilst procoagulant activity by the XaCT assay was higher (p=0.002). In smokers (n=21), certain MV subsets (CD41, TF and PS) and functional activity (ETP) was lower (p<0.05). Regression analysis showed that MV parameters of CD41, CD105, TF and ETP could be predicted independently by age, whilst smoking predicted for CD105, CD235, TF, PS and ETP. Certain MV parameters also correlated with BMI, lipid and hormone levels. The small RNA and miRNA levels did not differ by age group, smoking status or gender. CONCLUSIONS: It is important to recognize that differences may arise depending on age, gender, BMI, lipid, hormone levels and smoking status in apparently healthy subjects when evaluating MV for pathogenic potential.
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Authors: Ursula S Sandau; Erika Duggan; Xiao Shi; Sierra J Smith; Marilyn Huckans; William E Schutzer; Jennifer M Loftis; Aaron Janowsky; John P Nolan; Julie A Saugstad Journal: J Extracell Vesicles Date: 2020-11-28