Literature DB >> 2860085

Application of avidin-ferritin and peroxidase as contrasting electron-dense markers for simultaneous electron microscopic immunocytochemical labelling of glutamic acid decarboxylase and tyrosine hydroxylase in the rat arcuate nucleus.

C Leranth, H Sakamoto, N J MacLusky, M Shanabrough, F Naftolin.   

Abstract

A pre-embedding immunostaining procedure was developed using ferritin and peroxidase to enable simultaneous electron microscopic localization of two antigens in the same tissue section. This method was used to study the anatomic relationship between glutamic acid decarboxylase (GAD) immunoreactive axons and tyrosine hydroxylase (TH) - containing neurons of the rat arcuate nucleus. The findings provide ultrastructural evidence that GAD-immunoreactive terminals establish symmetric (Gray II) synapses on TH-reactive neurons.

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Year:  1985        PMID： 2860085     DOI： 10.1007/bf00708201

Source DB:  PubMed          Journal:  Histochemistry        ISSN： 0301-5564


  17 in total

1.  Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures.

Authors:  S M Hsu; L Raine; H Fanger
Journal:  J Histochem Cytochem       Date:  1981-04       Impact factor: 2.479

2.  Commissural afferents innervate glutamate decarboxylase immunoreactive non-pyramidal neurons in the guinea pig hippocampus.

Authors:  M Frotscher; C Léránth; K Lübbers; W H Oertel
Journal:  Neurosci Lett       Date:  1984-05-04       Impact factor: 3.046

3.  Evidence for noradrenergic mediation of opioid effects on luteinizing hormone secretion.

Authors:  S P Kalra; J W Simpkins
Journal:  Endocrinology       Date:  1981-09       Impact factor: 4.736

4.  The preparation of protein A-gold complexes with 3 nm and 15nm gold particles and their use in labelling multiple antigens on ultra-thin sections.

Authors:  J Roth
Journal:  Histochem J       Date:  1982-09

5.  Ultrastructural localization of tyrosine hydroxylase in noradrenergic neurons of brain.

Authors:  V M Pickel; T H Joh; D J Reis
Journal:  Proc Natl Acad Sci U S A       Date:  1975-02       Impact factor: 11.205

6.  Synaptology and sources of vasoactive intestinal polypeptide and substance P containing axons of the cat celiac ganglion. An experimental electron microscopic immunohistochemical study.

Authors:  C Léránth; E Fehér
Journal:  Neuroscience       Date:  1983-11       Impact factor: 3.590

7.  The section-Golgi impregnation procedure. 2. Immunocytochemical demonstration of glutamate decarboxylase in Golgi-impregnated neurons and in their afferent synaptic boutons in the visual cortex of the cat.

Authors:  P Somogyi; T F Freund; J Y Wu; A D Smith
Journal:  Neuroscience       Date:  1983-07       Impact factor: 3.590

8.  Involvement of preoptic-anterior hypothalamic GABA neurons in the regulation of pituitary LH and prolactin release.

Authors:  R Lamberts; E Vijayan; M Graf; T Mansky; W Wuttke
Journal:  Exp Brain Res       Date:  1983       Impact factor: 1.972

9.  Catecholamine turnover rates in discrete hypothalamic areas and associated changes in median eminence luteinizing hormone-releasing hormone and serum gonadotropins on proestrus and diestrous day 1.

Authors:  N Rance; P M Wise; M K Selmanoff; C A Barraclough
Journal:  Endocrinology       Date:  1981-05       Impact factor: 4.736

10.  Use of colloidal gold particles in double-labeling immunoelectron microscopy of ultrathin frozen tissue sections.

Authors:  H J Geuze; J W Slot; P A van der Ley; R C Scheffer
Journal:  J Cell Biol       Date:  1981-06       Impact factor: 10.539
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  7 in total

1.  Two different types of dynorphin-A-immunoreactive terminals in rat substantia nigra.

Authors:  R Riesenberg; C Nitsch
Journal:  Cell Tissue Res       Date:  1990-07       Impact factor: 5.249

2.  Simultaneous detection of indoleamines and dopamine in rat dorsal raphe nuclei using specific antibodies.

Authors:  M Geffard; S Tuffet; N Mons; J L Chagnaud
Journal:  Histochemistry       Date:  1987

3.  GABAergic input of cholecystokinin-immunoreactive neurons in the hilar region of the rat hippocampus. An electron microscopic double immunostaining study.

Authors:  C Leranth; M Frotscher
Journal:  Histochemistry       Date:  1987

4.  Adrenergic innervation of corticotropin releasing factor (CRF)-synthesizing neurons in the hypothalamic paraventricular nucleus of the rat. A combined light and electron microscopic immunocytochemical study.

Authors:  Z Liposits; C Phelix; W K Paull
Journal:  Histochemistry       Date:  1986

5.  Catecholaminergic innervation of pyramidal and GABAergic nonpyramidal neurons in the rat hippocampus. Double label immunostaining with antibodies against tyrosine hydroxylase and glutamate decarboxylase.

Authors:  M Frotscher; C Leranth
Journal:  Histochemistry       Date:  1988

6.  A combined light and electron microscopic immunocytochemical method for the simultaneous localization of multiple tissue antigens. Tyrosine hydroxylase immunoreactive innervation of corticotropin releasing factor synthesizing neurons in the paraventricular nucleus of the rat.

Authors:  Z Liposits; D Sherman; C Phelix; W K Paull
Journal:  Histochemistry       Date:  1986

7.  Effect of infusing gamma-aminobutyric acid receptor agonists and antagonists into the medial preoptic area and ventromedial hypothalamus on prolactin secretion in male sheep.

Authors:  S A Ferreira; D A Browning; C J Scott; D E Kuehl; G L Jackson
Journal:  Endocrine       Date:  1998-12       Impact factor: 3.925
  7 in total

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