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Literature DB >> 2449405

Simultaneous detection of indoleamines and dopamine in rat dorsal raphe nuclei using specific antibodies.

M Geffard¹, S Tuffet, N Mons, J L Chagnaud.

Abstract

Using a monoclonal antibody against dopamine and a rabbit antiserum against serotonin, 5-methoxytryptamine or tryptamine, we were able to achieve the simultaneous localization of two amines in glutaraldehyde-fixed sections of rat dorsal raphe nuclei. In this staining procedure, the first antigen was localized using 3,3'-diaminobenzidine (DAB), while the second antigen was stained using the 1-naphthol basic dye (2-NBD) method. The two antigens were localized in different cells or structures. No overlap of the staining was observed, thus indicating that dopamine is not localized with serotonin, 5-methoxytryptamine or tryptamine.

Entities: Chemical Species

Mesh：

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Year: 1987 PMID： 2449405 DOI： 10.1007/bf00490169

Source DB: PubMed Journal: Histochemistry ISSN： 0301-5564

18 in total

1. Antisera against small neurotransmitter-like molecules.

Authors: M Geffard; A M Henrich-Rock; J Dulluc; P Seguela
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2. One perfusion mixture for immunocytochemical detection of noradrenaline, dopamine, serotonin and acetylcholine in the same rat brain.

Authors: A McRae-Degueurce; M Geffard
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3. Antibodies to dopamine: radioimmunological study of specificity in relation to immunocytochemistry.

Authors: M Geffard; O Kah; B Onteniente; P Seguela; M Le Moal; M Delaage
Journal: J Neurochem Date: 1984-06 Impact factor: 5.372

4. Immunohistochemical studies on the localization and distribution of monoamine neuron systems in the rat brain. I. Tyrosine hydroxylase in the mes- and diencephalon.

Authors: T Hökfelt; O Johansson; K Fuxe; M Goldstein; D Park
Journal: Med Biol Date: 1976-12

5. Serotoninergic endings on VIP-neurons in the suprachiasmatic nucleus and on ACTH-neurons in the arcuate nucleus of the rat hypothalamus. A combination of high resolution autoradiography and electron microscopic immunocytochemistry.

Authors: J Kiss; C Léránth; B Halász
Journal: Neurosci Lett Date: 1984-02-10 Impact factor: 3.046

6. Comparison of serotonin and 5-methoxytryptamine immunoreactivity in rat raphe nuclei.

Authors: S Patel; J Dulluc; M Geffard
Journal: Histochemistry Date: 1986

7. Simultaneous and successive localization of two antigens in the same tissue section using 3,3'-diaminobenzidine and 1-naphthol basic dye.

Authors: A Mauro; A Bulfone; G Giaccone; D Schiffer
Journal: Histochemistry Date: 1986

8. 1-Naphthol basic dye (1-NBD). An alternative to diaminobenzidine (DAB) in immunoperoxidase techniques.

Authors: A Mauro; I Germano; G Giaccone; M T Giordana; D Schiffer
Journal: Histochemistry Date: 1985

9. Silver-intensified gold and peroxidase as dual ultrastructural immunolabels for pre- and postsynaptic neurotransmitters.

Authors: A N van den Pol
Journal: Science Date: 1985-04-19 Impact factor: 47.728

10. Antisera against the indolealkylamines: tryptophan, 5-hydroxytryptophan, 5-hydroxytryptamine, 5-methoxytryptophan, and 5-methoxytryptamine tested by an enzyme-linked immunosorbent assay method.

Authors: M Geffard; J Dulluc; A M Rock
Journal: J Neurochem Date: 1985-04 Impact factor: 5.372

3 in total