Hai-Tao Yu1, Xiao-Yi Fu1, Bing Liang2, Shuang Wang3, Jian-Kang Liu4, Shu-Ran Wang5, Zhi-Hui Feng6. 1. School of Public Health, Jilin Medical University, 5 Jilin Street, FengMan District, Jilin City, 132013, Jilin, China. 2. HaiNan Branch of P.L.A. General Hospital, Sanya, 572014, Hainan, China. 3. Center for Disease Control and Prevention, TongZhou, Beijing, 101100, China. 4. Center for Mitochondrial Biology and Medicine, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology and Frontier Institute of Science and Technology, Xi'an Jiaotong University, 28 W. Xian-ning Road, Xi'an, 710049, Shanxi, China. 5. School of Public Health, Jilin Medical University, 5 Jilin Street, FengMan District, Jilin City, 132013, Jilin, China. shuranwang2014@163.com. 6. Center for Mitochondrial Biology and Medicine, The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology and Frontier Institute of Science and Technology, Xi'an Jiaotong University, 28 W. Xian-ning Road, Xi'an, 710049, Shanxi, China. zhfeng@mail.xjtu.edu.cn.
Abstract
PURPOSE: Mitochondrial dysfunction plays an important role in the development of obesity and obesity-associated metabolic diseases. METHODS: In this study, we dynamically observed the characteristics of mitochondrial damage in a rat model of diet-induced obesity (DIO). From the 2nd to the 10th week, animals were killed every 2 weeks and the heart, liver, kidney, and testicular tissues were harvested. Mitochondria were isolated and the activities of respiratory chain complexes I, II, III, and IV as well as the 8-Hydroxy-2-deoxy Guanosine content were determined. Reactive oxygen species and malondialdehyde were measured. RESULTS: Mitochondrial damages were observed in the heart and liver of DIO and DR rats, and the damages occurred later in DR group than that in DIO group. The mitochondrial membrane potential of heart and liver decreased in DIO and DR groups. The activity of the heart mitochondria complexes I, III, and IV (composing NADH oxidative respiratory) was higher in the early stage of DIO and lower in the end of week 10. The higher activity of the liver complexes I, III, and IV was found until the end of week 10 in DIO and DR groups, accompanied with enhanced oxidative stress. Besides, mitochondrial DNA damages were observed in all tissues. CONCLUSION: In DIO rats, the heart mitochondrial dysfunction occurred first and the liver presented the strongest compensatory ability against oxidative stress.
PURPOSE:Mitochondrial dysfunction plays an important role in the development of obesity and obesity-associated metabolic diseases. METHODS: In this study, we dynamically observed the characteristics of mitochondrial damage in a rat model of diet-induced obesity (DIO). From the 2nd to the 10th week, animals were killed every 2 weeks and the heart, liver, kidney, and testicular tissues were harvested. Mitochondria were isolated and the activities of respiratory chain complexes I, II, III, and IV as well as the 8-Hydroxy-2-deoxy Guanosine content were determined. Reactive oxygen species and malondialdehyde were measured. RESULTS: Mitochondrial damages were observed in the heart and liver of DIO and DR rats, and the damages occurred later in DR group than that in DIO group. The mitochondrial membrane potential of heart and liver decreased in DIO and DR groups. The activity of the heart mitochondria complexes I, III, and IV (composing NADH oxidative respiratory) was higher in the early stage of DIO and lower in the end of week 10. The higher activity of the liver complexes I, III, and IV was found until the end of week 10 in DIO and DR groups, accompanied with enhanced oxidative stress. Besides, mitochondrial DNA damages were observed in all tissues. CONCLUSION: In DIO rats, the heart mitochondrial dysfunction occurred first and the liver presented the strongest compensatory ability against oxidative stress.
Authors: Mark E Pepin; Christoph Koentges; Katharina Pfeil; Johannes Gollmer; Sophia Kersting; Sebastian Wiese; Michael M Hoffmann; Katja E Odening; Constantin von Zur Mühlen; Philipp Diehl; Peter Stachon; Dennis Wolf; Adam R Wende; Christoph Bode; Andreas Zirlik; Heiko Bugger Journal: Front Endocrinol (Lausanne) Date: 2019-12-13 Impact factor: 5.555