Cristina Canal1, Raul Fontelo2, Ines Hamouda2, Jordi Guillem-Marti3, Uros Cvelbar4, Maria-Pau Ginebra5. 1. Biomaterials, Biomechanics and Tissue Engineering Group, Dpt. Materials Science and Metallurgy, Technical University of Catalonia (UPC), c. Eduard Maristany 10-14, 08019 Barcelona, Spain; Barcelona Research Center in Multiscale Science and Engineering, Universitat Politècnica de Catalunya, Barcelona, Spain. Electronic address: cristina.canal@upc.edu. 2. Biomaterials, Biomechanics and Tissue Engineering Group, Dpt. Materials Science and Metallurgy, Technical University of Catalonia (UPC), c. Eduard Maristany 10-14, 08019 Barcelona, Spain. 3. Biomaterials, Biomechanics and Tissue Engineering Group, Dpt. Materials Science and Metallurgy, Technical University of Catalonia (UPC), c. Eduard Maristany 10-14, 08019 Barcelona, Spain; Barcelona Research Center in Multiscale Science and Engineering, Universitat Politècnica de Catalunya, Barcelona, Spain. 4. Department of Surface Engineering and Optoelectronics (F-4), Jožef Stefan Institute, Jamova cesta 39, 1000 Ljubljana, Slovenia. 5. Biomaterials, Biomechanics and Tissue Engineering Group, Dpt. Materials Science and Metallurgy, Technical University of Catalonia (UPC), c. Eduard Maristany 10-14, 08019 Barcelona, Spain; Barcelona Research Center in Multiscale Science and Engineering, Universitat Politècnica de Catalunya, Barcelona, Spain; Institute for Bioengineering of Catalonia, c/ Baldiri i Reixach 10-12, 08028 Barcelona, Spain.
Abstract
BACKGROUND: Current therapies for bone cancers - either primary or metastatic - are difficult to implement and unfortunately not completely effective. An alternative therapy could be found in cold plasmas generated at atmospheric pressure which have already demonstrated selective anti-tumor action in a number of carcinomas and in more relatively rare brain tumors. However, its effects on bone cancer are still unknown. METHODS: Herein, we employed an atmospheric pressure plasma jet (APPJ) to validate its selectivity towards osteosarcoma cell line vs. osteoblasts & human mesenchymal stem cells. RESULTS: Cytotoxicity following direct interaction of APPJ with cells is comparable to indirect interaction when only liquid medium is treated and subsequently added to the cells, especially on the long-term (72h of cell culture). Moreover, following contact of the APPJ treated medium with cells, delayed effects are observed which lead to 100% bone cancer cell death through apoptosis (decreased cell viability with incubation time in contact with APPJ treated medium from 24h to 72h), while healthy cells remain fully viable and unaffected by the treatment. CONCLUSIONS: The high efficiency of the indirect treatment indicates that an important role is played by the reactive oxygen species (ROS) and reactive nitrogen species (RNS) in the gaseous plasma stage and then transmitted to the liquid phase, which overall lead to lethal and selective action towards osteosarcoma cells. These findings open new pathways for treatment of metastatic bone disease with a minimally invasive approach.
BACKGROUND: Current therapies for bone cancers - either primary or metastatic - are difficult to implement and unfortunately not completely effective. An alternative therapy could be found in cold plasmas generated at atmospheric pressure which have already demonstrated selective anti-tumor action in a number of carcinomas and in more relatively rare brain tumors. However, its effects on bone cancer are still unknown. METHODS: Herein, we employed an atmospheric pressure plasma jet (APPJ) to validate its selectivity towards osteosarcoma cell line vs. osteoblasts & human mesenchymal stem cells. RESULTS:Cytotoxicity following direct interaction of APPJ with cells is comparable to indirect interaction when only liquid medium is treated and subsequently added to the cells, especially on the long-term (72h of cell culture). Moreover, following contact of the APPJ treated medium with cells, delayed effects are observed which lead to 100% bone cancer cell death through apoptosis (decreased cell viability with incubation time in contact with APPJ treated medium from 24h to 72h), while healthy cells remain fully viable and unaffected by the treatment. CONCLUSIONS: The high efficiency of the indirect treatment indicates that an important role is played by the reactive oxygen species (ROS) and reactive nitrogen species (RNS) in the gaseous plasma stage and then transmitted to the liquid phase, which overall lead to lethal and selective action towards osteosarcoma cells. These findings open new pathways for treatment of metastatic bone disease with a minimally invasive approach.
Authors: Josephine M Jacoby; Silas Strakeljahn; Andreas Nitsch; Sander Bekeschus; Peter Hinz; Alexander Mustea; Axel Ekkernkamp; Mladen V Tzvetkov; Lyubomir Haralambiev; Matthias B Stope Journal: Int J Mol Sci Date: 2020-06-23 Impact factor: 5.923
Authors: F Riedel; J Golda; J Held; H L Davies; M W van der Woude; J Bredin; K Niemi; T Gans; V Schulz-von der Gathen; D O'Connell Journal: Plasma Sources Sci Technol Date: 2020-09-17 Impact factor: 3.584