Suganthi Martena Devadas1, Mamatha Ballal2, Peralam Yegneswaran Prakash3, Manjunath H Hande4, Geetha V Bhat5, Vinitha Mohandas6. 1. Research Scholar, Department of Enteric Diseases Division, KMC, Manipal University, Manipal, Karnataka, India. 2. Professor, Department of Enteric Diseases Division, KMC, Manipal University, Manipal, Karnataka, India. 3. Associate Professor and Incharge-Medical Mycology Laboratory, Department of Microbiology, KMC, Manipal University, Manipal, Karnataka, India. 4. Professor and Head, Department of Medicine, KMC, Manipal University, Manipal, Karnataka, India. 5. Microbiologist and Infection Control Officer, Department of Microbiology, Bhagwan Mahaveer Jain Hospital, Bengaluru, Karnataka, India. 6. Professor, Department of Microbiology, Bangalore Institute of Dental Sciences, Bengaluru, Karnataka, India.
Abstract
INTRODUCTION: The auxanographic carbohydrate assimilation had been an important method for differentiation of yeasts. Prevailing methods described in the literature for carbohydrate assimilation has limited scope for use in large scale yeast identification. AIM: To optimize the large scale auxanographic carbohydrate assimilation method for yeast identification. MATERIALS AND METHODS: A modified auxanographic carbohydrate assimilation method was developed and a total of 35 isolates of Candida species comprising of four ATCC (American Type Culture Collection) Candida strains (Candida albicans ATCC 90028, Candida tropicalis ATCC 90018, Candida parapsilosis ATCC 750, Candida krusei ATCC 6258) and 31 clinical isolates of Candida tropicalis (n=13), Candida krusei (n=7), Candida glabrata (n=3), Candida kefyr (n=3), Candida albicans (n=5) were validated. The carbohydrates tested were Glucose, Sucrose, Maltose, Lactose, Cellubiose, Raffinose, Trehalose, Xylose, Galactose and Dulcitol. RESULTS: A total of 35 Candida species were tested for their carbohydrate assimilative property and the results were consistent with the existing standard protocols. A well circumscribed opaque yeast growth indicated assimilation of the test carbohydrate and translucent to opalescent growth with the outline of initial inoculum alone indicated lack of assimilation. The control plate indicated no growth of the Candida species. CONCLUSION: The carbohydrate assimilation tests finds utility for yeast diversity studies exploring novel ecological niches. The technique described here facilitates testing of an extended range of carbohydrates and yeasts in a cost effective manner.
INTRODUCTION: The auxanographic carbohydrate assimilation had been an important method for differentiation of yeasts. Prevailing methods described in the literature for carbohydrate assimilation has limited scope for use in large scale yeast identification. AIM: To optimize the large scale auxanographic carbohydrate assimilation method for yeast identification. MATERIALS AND METHODS: A modified auxanographic carbohydrate assimilation method was developed and a total of 35 isolates of Candida species comprising of four ATCC (American Type Culture Collection) Candida strains (Candida albicans ATCC 90028, Candida tropicalis ATCC 90018, Candida parapsilosisATCC 750, Candida kruseiATCC 6258) and 31 clinical isolates of Candida tropicalis (n=13), Candida krusei (n=7), Candida glabrata (n=3), Candida kefyr (n=3), Candida albicans (n=5) were validated. The carbohydrates tested were Glucose, Sucrose, Maltose, Lactose, Cellubiose, Raffinose, Trehalose, Xylose, Galactose and Dulcitol. RESULTS: A total of 35 Candida species were tested for their carbohydrate assimilative property and the results were consistent with the existing standard protocols. A well circumscribed opaque yeast growth indicated assimilation of the test carbohydrate and translucent to opalescent growth with the outline of initial inoculum alone indicated lack of assimilation. The control plate indicated no growth of the Candida species. CONCLUSION: The carbohydrate assimilation tests finds utility for yeast diversity studies exploring novel ecological niches. The technique described here facilitates testing of an extended range of carbohydrates and yeasts in a cost effective manner.
Entities:
Keywords:
Candida; Control plate; Ecological niche
Authors: Geraldo dos Santos Oliveira; Elizabeth Teixeira Ribeiro; Francisco de Assis Baroni Journal: Rev Inst Med Trop Sao Paulo Date: 2006 Nov-Dec Impact factor: 1.846
Authors: Mariana Castanheira; Leah N Woosley; Daniel J Diekema; Ronald N Jones; Michael A Pfaller Journal: J Clin Microbiol Date: 2012-10-24 Impact factor: 5.948
Authors: Ratna Mohd Tap; Lee Sue Ho Betty; Nur Yasmin Ramli; Jeyanthi Suppiah; Rohaidah Hashim; Parameswari Sabaratnam; Stephanie Jane Ginsapu; Annabel Gowbei; Mohd Fuat Abd Razak; Matthias Sipiczki; Norazah Ahmad Journal: Mycoses Date: 2016-11 Impact factor: 4.377