LIMITS TO THE SOUTHERN BORDER OF DROSOPHILA SERRATA: COLD RESISTANCE, HERITABLE VARIATION, AND TRADE-OFFS.

There are a number of evolutionary hypotheses about why species distributions are limited, but very little empirical information to test them. We present data examining whether the southern distribution of Drosophila serrata is limited by cold responses. Species comparisons were undertaken for cold resistance, development time, and viability at 15°C and 25°C for D. serrata and other species with a more southerly distribution (D. melanogaster, D. simulans, and D. immigrans). Relative to the other species, D. serrata had a long development time at both temperatures and a low level of cold resistance. Using isofemale lines collected in different seasons, central and marginal populations were compared for cold resistance, as well as development time and viability at 14°C. The border population had a relatively higher resistance to cold shock in postwinter collections, but there was no population differentiation for prewinter collections or for the other traits. The presence of variation among isofemale lines within the border populations suggests that genetic variation as measured in the laboratory is unlikely to limit range expansion. Population cages were used in the field to determine if D. serrata persisted over winter at borders. Although all cages yielded adult offspring at northern sites, only a few produced offspring at or just south of the border. In contrast, all cages with D. simulans produced adult offspring, suggesting that climatic factors limited D. serrata numbers. Offspring from surviving adults showed a phenotypic trade-off between fecundity and cold resistance. Comparisons of the cold resistance of field males and females with their laboratory-reared offspring provided evidence for heritable variation in field-reared flies. Overall, the results suggest that cold stress is important in limiting the southern distribution of D. serrata, but it seems unlikely that a lack of genetic variation restricts range expansion. © 1999 The Society for the Study of Evolution.