Blanca Torrejón1, Ion Cristóbal2, Federico Rojo3, Jesús García-Foncillas4. 1. Translational Oncology Division, Oncohealth Institute, IIS-Fundación Jiménez Díaz, UAM, University Hospital "Fundación Jiménez Díaz", Avda. Reyes Católicos-2, 28040, Madrid, Spain. 2. Translational Oncology Division, Oncohealth Institute, IIS-Fundación Jiménez Díaz, UAM, University Hospital "Fundación Jiménez Díaz", Avda. Reyes Católicos-2, 28040, Madrid, Spain. ion.cristobal@fjd.es. 3. Pathology Department, University Hospital "Fundacion Jimenez Diaz", Autonomous University of Madrid, 28040, Madrid, Spain. 4. Translational Oncology Division, Oncohealth Institute, IIS-Fundación Jiménez Díaz, UAM, University Hospital "Fundación Jiménez Díaz", Avda. Reyes Católicos-2, 28040, Madrid, Spain. jgfoncillas@fjd.es.
Abstract
BACKGROUND: Caveolin-1 (CAV-1), the main scaffold protein in caveolae, is frequently deregulated in human cancer. Of importance, this protein has been described to show tumor suppressor or oncogenic properties depending on the cell type and the stage of the disease. In fact, its role in colorectal cancer (CRC) remains to be fully clarified due to discrepancies in the literature. METHODS: We analyzed CAV-1 by western blot in a set of early-stage CRC patients with paired tumor tissue and normal colonic mucosa available. CAV-1 mRNA and expression levels of miR-124, 133 and 802 were quantified by real-time PCR. RESULTS: We found CAV-1 strongly downregulated in 76.2% of tumor samples and associated with the subgroup of elderly patients (p = 0.027). We observed by real-time PCR a lack of correlation between CAV-1 mRNA and protein levels in some cases with CAV-1 downregulated by western blot, and miR-124 deregulation was identified as a potential contributing alteration to decrease CAV-1 protein expression. CONCLUSION: CAV-1 is commonly downregulated in patients with primary CRC, which suggests its tumor suppressor role in early stages of this disease. Moreover, based on our findings, the previous discrepancies observed in different studies to date could be due to a complex posttranscriptional CAV-1 regulation.
BACKGROUND:Caveolin-1 (CAV-1), the main scaffold protein in caveolae, is frequently deregulated in humancancer. Of importance, this protein has been described to show tumor suppressor or oncogenic properties depending on the cell type and the stage of the disease. In fact, its role in colorectal cancer (CRC) remains to be fully clarified due to discrepancies in the literature. METHODS: We analyzed CAV-1 by western blot in a set of early-stage CRC patients with paired tumor tissue and normal colonic mucosa available. CAV-1 mRNA and expression levels of miR-124, 133 and 802 were quantified by real-time PCR. RESULTS: We found CAV-1 strongly downregulated in 76.2% of tumor samples and associated with the subgroup of elderly patients (p = 0.027). We observed by real-time PCR a lack of correlation between CAV-1 mRNA and protein levels in some cases with CAV-1 downregulated by western blot, and miR-124 deregulation was identified as a potential contributing alteration to decrease CAV-1 protein expression. CONCLUSION:CAV-1 is commonly downregulated in patients with primary CRC, which suggests its tumor suppressor role in early stages of this disease. Moreover, based on our findings, the previous discrepancies observed in different studies to date could be due to a complex posttranscriptional CAV-1 regulation.
Authors: Murat Cokakli; Esra Erdal; Deniz Nart; Funda Yilmaz; Ozgul Sagol; Murat Kilic; Sedat Karademir; Nese Atabey Journal: BMC Cancer Date: 2009-02-24 Impact factor: 4.430