BACKGROUND: Caveolin-1 has been shown to be down-regulated in human colon cancer and involved in colon tumorigenesis. We investigated the mechanism. MATERIALS AND METHODS: Cancerous and nearby non-cancerous tissues of 185 sporadic colorectal cancer samples were enrolled in this study. Methylation-specific PCR was performed to explore the mechanism of regulation of caveolin-1 gene expression. RESULTS: Aberrant promoter methylation in the caveolin-1 gene was 3.8% and 5.9% for cancerous and nearby non-cancerous tissues, respectively. All the cancerous and non-cancerous tissue contained unmethylated promoters in the caveolin-1 gene. The methylation status of caveolin-1 had no clear relationship with age, cell grade, location of tumor or lymph node metastasis. However, female gender showed statistically significant difference (p=0.045). The immunohistochemistry study demonstrated that expression of caveolin-1 correlated with aberrant promoter methylation status in sporadic colorectal cancer tissues. CONCLUSION: Our findings suggested that aberrant promoter methylation of the caveolin-1 gene may occur at the precancerous stage, regulated by gender-related factors and is associated with gene silencing of caveolin-1 in the development of colorectal cancer.
BACKGROUND:Caveolin-1 has been shown to be down-regulated in humancolon cancer and involved in colon tumorigenesis. We investigated the mechanism. MATERIALS AND METHODS:Cancerous and nearby non-cancerous tissues of 185 sporadic colorectal cancer samples were enrolled in this study. Methylation-specific PCR was performed to explore the mechanism of regulation of caveolin-1 gene expression. RESULTS: Aberrant promoter methylation in the caveolin-1 gene was 3.8% and 5.9% for cancerous and nearby non-cancerous tissues, respectively. All the cancerous and non-cancerous tissue contained unmethylated promoters in the caveolin-1 gene. The methylation status of caveolin-1 had no clear relationship with age, cell grade, location of tumor or lymph node metastasis. However, female gender showed statistically significant difference (p=0.045). The immunohistochemistry study demonstrated that expression of caveolin-1 correlated with aberrant promoter methylation status in sporadic colorectal cancer tissues. CONCLUSION: Our findings suggested that aberrant promoter methylation of the caveolin-1 gene may occur at the precancerous stage, regulated by gender-related factors and is associated with gene silencing of caveolin-1 in the development of colorectal cancer.
Authors: Bo Song; Jian-Wu Tang; Bo Wang; Xiao-Nan Cui; Li Hou; Lu Sun; Li-Min Mao; Chun-Hui Zhou; Yue Du; Li-Hui Wang; Hua-Xin Wang; Ren-Shu Zheng; Lei Sun Journal: World J Gastroenterol Date: 2005-03-14 Impact factor: 5.742
Authors: Elke Prade; Moritz Tobiasch; Ivana Hitkova; Isabell Schäffer; Fan Lian; Xiangbin Xing; Marc Tänzer; Sandra Rauser; Axel Walch; Marcus Feith; Stefan Post; Christoph Röcken; Roland M Schmid; Matthias P A Ebert; Elke Burgermeister Journal: Mol Endocrinol Date: 2012-04-03
Authors: Maria J Worsham; Josena K Stephen; Kang Mei Chen; Shaleta Havard; Veena Shah; Glendon Gardner; Vanessa G Schweitzer Journal: Cancer Lett Date: 2012-03-01 Impact factor: 8.679
Authors: Karin A Sloan; Hector A Marquez; Jun Li; Yuxia Cao; Anne Hinds; Carl J O'Hara; Satinder Kathuria; Maria I Ramirez; Mary C Williams; Hasmeena Kathuria Journal: Carcinogenesis Date: 2009-05-29 Impact factor: 4.944