| Literature DB >> 28554521 |
Zhongxue Dai1, Honglian Gu2, Shangjie Zhang3, Fengxue Xin4, Wenming Zhang5, Weiliang Dong6, Jiangfeng Ma7, Honghua Jia8, Min Jiang9.
Abstract
The aim of this study was to metabolically construct Saccharomyces cerevisiae for achievement of direct methanol utilization and value added product (mainly pyruvate) production. After successful integration of methanol oxidation pathway originated from Pichia pastoris into the chromosome of S. cerevisiae, the recombinant showed 1.04g/L consumption of methanol and 3.13% increase of cell growth (OD600) when using methanol as the sole carbon source. Moreover, 0.26g/L of pyruvate was detected in the fermentation broth. The supplementation of 1g/L yeast extract could further improve cell growth with increase of 11.70% and methanol consumption to 2.35g/L. This represents the first genetically modified non-methylotrophic eukaryotic microbe for direct methanol utilization and would be of great value concerning the development of biotechnological processes.Entities:
Keywords: Methanol; Non-methylotrophy; Pyruvate; S. cerevisiae; Synthetic biology
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Year: 2017 PMID： 28554521 DOI： 10.1016/j.biortech.2017.05.100
Source DB: PubMed Journal: Bioresour Technol ISSN： 0960-8524 Impact factor: 9.642