Literature DB >> 28552427

EYK1 encoding erythrulose kinase as a catabolic selectable marker for genome editing in the non-conventional yeast Yarrowia lipolytica.

Marie Vandermies1, Olivia Denies1, Jean-Marc Nicaud2, Patrick Fickers3.   

Abstract

We report here on EYK1, encoding erythrulose kinase, as an efficient catabolic selectable marker for genome editing in Y. lipolytica. Compared to auxotrophic markers, EYK1 increases the growth rate of transformants and allows improved efficiency of transformation. The utility of the marker EYK1 in a replicative vector was also demonstrated.
Copyright © 2017 Elsevier B.V. All rights reserved.

Entities:  

Keywords:  Catabolic selectable marker; EYK1; Genome editing; Yarrowia lipolytica

Mesh：

Substances：

Year:  2017        PMID： 28552427     DOI： 10.1016/j.mimet.2017.05.012

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  5 in total

1.  New inducible promoter for gene expression and synthetic biology in Yarrowia lipolytica.

Authors:  Marion Trassaert; Marie Vandermies; Fréderic Carly; Olivia Denies; Stéphane Thomas; Patrick Fickers; Jean-Marc Nicaud
Journal:  Microb Cell Fact       Date:  2017-08-15       Impact factor: 5.328

Review 2.  Synthetic biology tools for engineering Yarrowia lipolytica.

Authors:  M Larroude; T Rossignol; J-M Nicaud; R Ledesma-Amaro
Journal:  Biotechnol Adv       Date:  2018-10-11       Impact factor: 14.227

3.  Efficient expression vectors and host strain for the production of recombinant proteins by Yarrowia lipolytica in process conditions.

Authors:  Young-Kyoung Park; Marie Vandermies; Paul Soudier; Samuel Telek; Stéphane Thomas; Jean-Marc Nicaud; Patrick Fickers
Journal:  Microb Cell Fact       Date:  2019-10-10       Impact factor: 5.328

4.  Identification of a Yarrowia lipolytica acetamidase and its use as a yeast genetic marker.

Authors:  Maureen Hamilton; Andrew L Consiglio; Kyle MacEwen; A Joe Shaw; Vasiliki Tsakraklides
Journal:  Microb Cell Fact       Date:  2020-02-05       Impact factor: 5.328

5.  A unique, newly discovered four-member protein family involved in extracellular fatty acid binding in Yarrowia lipolytica.

Authors:  Djamila Onésime; Léa Vidal; Stéphane Thomas; Céline Henry; Véronique Martin; Gwenaëlle André; Piotr Kubiak; Philippe Minard; Ewelina Celinska; Jean-Marc Nicaud
Journal:  Microb Cell Fact       Date:  2022-10-01       Impact factor: 6.352
  5 in total

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