Zhenhua Zhou1, Yan Li2, Xu Yan3, Xudong Wang1, Cheng Yang1, Haifeng Wei1, Xinghai Yang1, Jianru Xiao4. 1. Department of Orthopaedic Oncology, Changzheng Hospital, The Second Military Medical University, No.415, Fengyang Road, Shanghai, 200003, China. 2. Department of Oncology, Cancer Institute, Fudan University Shanghai Cancer Center, Shanghai Medical College, Fudan University, Shanghai, China. 3. Department of Orthopaedics, The 455th Hospital of PLA, Shanghai, China. 4. Department of Orthopaedic Oncology, Changzheng Hospital, The Second Military Medical University, No.415, Fengyang Road, Shanghai, 200003, China. jianruxiao63@126.com.
Abstract
PURPOSE: Osteosarcoma is one of the most common malignancies in bones and is often found in limbs. Until now, it is not clear why osteosarcoma is rare in the spine. On the other hand, previous biological characteristics study about osteosarcoma of spine was also rare because of its low incidence. To explore the biology of spinal osteosarcoma, a stable osteosarcoma cell line derived from spine is necessary. METHODS: A novel osteosarcoma cell line named NEO217 was established from spinal osteosarcoma tissues obtained from a Chinese male patient. We performed a series of experiments to investigate the biological properties of NEO217, including cell morphology, the kinetics of cell growth, biomarkers and tumorigenicity. RESULTS: The cell line NEO217 was passaged in vitro for more than 50 generations. Ultramicroscopic structural features of these cells were consistent with the pleomorphism characteristic of cancer cells. The average cell doubling time was 26 h. The chromosomal morphology was that of a human karyotype, with the number of chromosomes more than 80. NEO217 cells and available osteosarcoma cell lines such as MG-63 and MNNG/HOS were all CD29+CD59+ phenotype as detected by flow cytometry. Inoculation of NEO217 cells to immunodeficient mice led to tumor formation. The biological and molecular properties of NEO217 cell line are not exactly the same as some human osteosarcoma cell lines derived from the extremities. CONCLUSION: We have established a novel osteosarcoma cell line NEO217 derived from the spine, which will provide a useful model for biological or therapeutical studies of spinal osteosarcoma.
PURPOSE:Osteosarcoma is one of the most common malignancies in bones and is often found in limbs. Until now, it is not clear why osteosarcoma is rare in the spine. On the other hand, previous biological characteristics study about osteosarcoma of spine was also rare because of its low incidence. To explore the biology of spinal osteosarcoma, a stable osteosarcoma cell line derived from spine is necessary. METHODS: A novel osteosarcoma cell line named NEO217 was established from spinal osteosarcoma tissues obtained from a Chinese male patient. We performed a series of experiments to investigate the biological properties of NEO217, including cell morphology, the kinetics of cell growth, biomarkers and tumorigenicity. RESULTS: The cell line NEO217 was passaged in vitro for more than 50 generations. Ultramicroscopic structural features of these cells were consistent with the pleomorphism characteristic of cancer cells. The average cell doubling time was 26 h. The chromosomal morphology was that of a human karyotype, with the number of chromosomes more than 80. NEO217 cells and available osteosarcoma cell lines such as MG-63 and MNNG/HOS were all CD29+CD59+ phenotype as detected by flow cytometry. Inoculation of NEO217 cells to immunodeficientmice led to tumor formation. The biological and molecular properties of NEO217 cell line are not exactly the same as some humanosteosarcoma cell lines derived from the extremities. CONCLUSION: We have established a novel osteosarcoma cell line NEO217 derived from the spine, which will provide a useful model for biological or therapeutical studies of spinal osteosarcoma.
Authors: A Billiau; V G Edy; H Heremans; J Van Damme; J Desmyter; J A Georgiades; P De Somer Journal: Antimicrob Agents Chemother Date: 1977-07 Impact factor: 5.191
Authors: Jeanine L Van Nostrand; Alice Brisac; Stephano S Mello; Suzanne B R Jacobs; Richard Luong; Laura D Attardi Journal: Cancer Discov Date: 2015-03-26 Impact factor: 39.397
Authors: Alexander B Mohseny; Karoly Szuhai; Salvatore Romeo; Emilie P Buddingh; Inge Briaire-de Bruijn; Daniëlle de Jong; Melissa van Pel; Anne-Marie Cleton-Jansen; Pancras C W Hogendoorn Journal: J Pathol Date: 2009-11 Impact factor: 7.996