Marco Brayda-Bruno1, Marco Viganò2, Sabina Cauci3, Jacopo A Vitale4, Laura de Girolamo5, Paola De Luca6, Giovanni Lombardi7, Giuseppe Banfi8, Alessandra Colombini9. 1. Scoliosis Unit, Department of Orthopedics and Traumatology-Spine Surgery III, IRCCS Galeazzi Orthopaedic Institute, via R. Galeazzi 4, 20161 Milan, Italy. Electronic address: marco.brayda@spinecaregroup.it. 2. Orthopaedic Biotechnology Lab, IRCCS Galeazzi Orthopaedic Institute, via R. Galeazzi 4, 20161 Milan, Italy; Department of Biotechnology and Biosciences, University of Milano-Bicocca, P.zza della Scienza, 1, 20126 Milan, Italy. Electronic address: marco.vigano@grupposandonato.it. 3. Department of Medical Area, University of Udine, P.le Kolbe 4, 33100 Udine, Italy. Electronic address: sabina.cauci@uniud.it. 4. Laboratory of Biological Structure Mechanics, IRCCS Galeazzi Orthopaedic Institute, via R. Galeazzi 4, 20161 Milan, Italy. Electronic address: jacopo.vitale@grupposandonato.it. 5. Orthopaedic Biotechnology Lab, IRCCS Galeazzi Orthopaedic Institute, via R. Galeazzi 4, 20161 Milan, Italy. Electronic address: laura.degirolamo@grupposandonato.it. 6. Orthopaedic Biotechnology Lab, IRCCS Galeazzi Orthopaedic Institute, via R. Galeazzi 4, 20161 Milan, Italy. Electronic address: deluca.paola@grupposandonato.it. 7. Laboratory of Experimental Biochemistry & Molecular Biology, IRCCS Galeazzi Orthopaedic Institute, via R. Galeazzi 4, 20161 Milan, Italy. Electronic address: giovanni.lombardi@grupposandonato.it. 8. Vita-Salute San Raffaele University, Via Olgettina, 58, 20132 Milan, Italy. Electronic address: banfi.giuseppe@fondazionesanraffaele.it. 9. Orthopaedic Biotechnology Lab, IRCCS Galeazzi Orthopaedic Institute, via R. Galeazzi 4, 20161 Milan, Italy. Electronic address: alessandra.colombini@grupposandonato.it.
Abstract
OBJECTIVE: To evaluate plasma vitamin D and cross-linked C-telopeptides of type I (CTx-I) and type II (CTx-II) collagen concentrations in males with lumbar intervertebral disc degeneration (IVD) compared to healthy controls. Improved knowledge might suggest to optimize the vitamin D status of IVD patients and contribute to clarify mechanisms of cartilage degradation. METHODS: 79 Italian males with lumbar IVD assessed by Magnetic Resonance Imaging (MRI) and 79 age, sex and BMI-matched healthy controls were enrolled. Plasma 25hydroxyvitamin D (25(OH)D), CTx-I and CTx-II were measured by immunoassays. Circannual seasonality, correlation between biomarkers concentrations and clinical variables were assessed. RESULTS: Overall subjects 25(OH)D and CTx-II showed month rhythmicity with acrophase in August/September and October/November, and nadir in February/March and April/May, respectively. An inverse correlation between 25(OH)D and CTx-I, and a direct correlation between CTx-II and CTx-I were observed. IVD patients, particularly with osteochondrosis, showed higher CTx-II than healthy controls. CONCLUSIONS: Month of sampling may affect plasma 25(OH)D and CTx-II concentrations. The correlation between CTx-I and CTx-II suggests an interplay between the osteo-cartilaginous endplate and the fibro-cartilaginous disc. The results of this study highlighted that osteochondrosis associates with increased cartilaginous catabolism. Vitamin D supplementation seems more necessary in winter for lumbar IVD patients.
OBJECTIVE: To evaluate plasma vitamin D and cross-linked C-telopeptides of type I (CTx-I) and type II (CTx-II) collagen concentrations in males with lumbar intervertebral disc degeneration (IVD) compared to healthy controls. Improved knowledge might suggest to optimize the vitamin D status of IVDpatients and contribute to clarify mechanisms of cartilage degradation. METHODS: 79 Italian males with lumbar IVD assessed by Magnetic Resonance Imaging (MRI) and 79 age, sex and BMI-matched healthy controls were enrolled. Plasma 25hydroxyvitamin D (25(OH)D), CTx-I and CTx-II were measured by immunoassays. Circannual seasonality, correlation between biomarkers concentrations and clinical variables were assessed. RESULTS: Overall subjects 25(OH)D and CTx-II showed month rhythmicity with acrophase in August/September and October/November, and nadir in February/March and April/May, respectively. An inverse correlation between 25(OH)D and CTx-I, and a direct correlation between CTx-II and CTx-I were observed. IVDpatients, particularly with osteochondrosis, showed higher CTx-II than healthy controls. CONCLUSIONS: Month of sampling may affect plasma 25(OH)D and CTx-II concentrations. The correlation between CTx-I and CTx-II suggests an interplay between the osteo-cartilaginous endplate and the fibro-cartilaginous disc. The results of this study highlighted that osteochondrosis associates with increased cartilaginous catabolism. Vitamin D supplementation seems more necessary in winter for lumbar IVDpatients.
Authors: Paola De Luca; Laura de Girolamo; Carlotta Perucca Orfei; Marco Viganò; Riccardo Cecchinato; Marco Brayda-Bruno; Alessandra Colombini Journal: Int J Mol Sci Date: 2018-07-09 Impact factor: 5.923
Authors: Sabina Cauci; Marco Viganò; Laura de Girolamo; Paola De Luca; Carlotta Perucca Orfei; Giuseppe Banfi; Giovanni Lombardi; Marco Brayda-Bruno; Alessandra Colombini Journal: Int J Mol Sci Date: 2017-09-29 Impact factor: 5.923