Ayse Feyda Nursal1, Süheyla Kaya2, Ozlem Sezer3, Nevin Karakus4, Serbulent Yigit4. 1. Faculty of Medicine, Department of Medical Genetic, HititUniversity, Corum, Turkey. 2. Faculty of Medicine, Department of Internal Medicine, Gaziosmapasa University, Tokat, Turkey. 3. Deparment of Medical Genetics, Samsun Training and Research Hospital, Genetic Clinics, Samsun, Turkey. 4. Faculty of Medicine, Deparment of Medical Biology, Gaziosmanpasa University, Tokat, Turkey.
Abstract
BACKGROUND: Methylenetetrahydrofolate reductase (MTHFR) is a crucial enzyme in homocysteine (Hcy) metabolism. We aimed to evaluate a possible relationship between MTHFR gene C677T (rs 1801133), A1298C (rs 1801131) variants and susceptibility to FMF in a Turkish cohort. MATERIAL- METHODS: This case-control study included 198 Turkish FMF patients and 100 healthy subjects as controls. MTHFR C677T and A1298C were analyzed by polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) methods. RESULTS: The genotype distribution and allele frequency of the MTHFR C677T were statistically different between the patients and the control group (P=.006, P=.001, respectively). The frequency of the TT genotype and T allele of MTHFR C677T was significantly higher in the patients than in the controls. The genotype distribution of MTHFR A1298C variant did not show any statistically significant difference between the patients and the controls (P›.05). The patients had statistically different frequencies in allele C of MTHFR A1298C variant compared with the control (P=.032). We also examined the risk associated with inheriting the combined genotypes for the two MTHFR variants. According to these results, individuals who were CC homozygous at C677T locus and AA homozygous at A1298C locus have a lower risk of developing FMF (P=.002). Individuals who were TT homozygous at C677T locus and AC heterozygous at A1298C locus have higher risk of developing FMF (P=.033). CONCLUSION: Our findings clearly showed there was an association the MTHFR C677T/A1298C variants and susceptibility to FMF in the Turkish sample.
BACKGROUND:Methylenetetrahydrofolate reductase (MTHFR) is a crucial enzyme in homocysteine (Hcy) metabolism. We aimed to evaluate a possible relationship between MTHFR gene C677T (rs 1801133), A1298C (rs 1801131) variants and susceptibility to FMF in a Turkish cohort. MATERIAL- METHODS: This case-control study included 198 Turkish FMFpatients and 100 healthy subjects as controls. MTHFRC677T and A1298C were analyzed by polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) methods. RESULTS: The genotype distribution and allele frequency of the MTHFRC677T were statistically different between the patients and the control group (P=.006, P=.001, respectively). The frequency of the TT genotype and T allele of MTHFRC677T was significantly higher in the patients than in the controls. The genotype distribution of MTHFRA1298C variant did not show any statistically significant difference between the patients and the controls (P›.05). The patients had statistically different frequencies in allele C of MTHFRA1298C variant compared with the control (P=.032). We also examined the risk associated with inheriting the combined genotypes for the two MTHFR variants. According to these results, individuals who were CC homozygous at C677T locus and AA homozygous at A1298C locus have a lower risk of developing FMF (P=.002). Individuals who were TT homozygous at C677T locus and AC heterozygous at A1298C locus have higher risk of developing FMF (P=.033). CONCLUSION: Our findings clearly showed there was an association the MTHFRC677T/A1298C variants and susceptibility to FMF in the Turkish sample.
Authors: J David Symons; Adam E Mullick; Jodi L Ensunsa; Amy A Ma; John C Rutledge Journal: Arterioscler Thromb Vasc Biol Date: 2002-05-01 Impact factor: 8.311
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