| Literature DB >> 28542522 |
Wenyang Sun1,2, Jing Lan2, Lei Chen2, Jinjie Qiu2, Zonggang Luo2,3, Mingzhou Li4, Jinyong Wang2, Jiugang Zhao2, Tinghuan Zhang2, Xi Long2, Jie Chai2, Zunqiang Yan1,2, Zongyi Guo2, Shuangbao Gun1,5.
Abstract
MicroRNAs (miRNAs) are small non-coding RNAs that are involved in translational regulation of the messenger RNA molecules. Sequence variations in the genes encoding miRNAs could influence their biogenesis and function. MiR-15b plays an important role in cellular proliferation, apoptosis and the cell cycle. Here, we report the identification of a C58T mutation in porcine pre-miR-15b. Through in vitro and in vivo experiments, we determined that this mutation blocks the transition from pri-miRNA to pre-miRNA, alters the strand selection between miR-15b-5p and miR-15b-3p, and obstructs biogenesis of the downstream miR-16-1. These results serve to highlight the importance of miRNA mutations and their impacts on miRNA biogenesis.Entities:
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Year: 2017 PMID： 28542522 PMCID： PMC5443575 DOI： 10.1371/journal.pone.0178045
Source DB: PubMed Journal: PLoS One ISSN： 1932-6203 Impact factor: 3.240