| Literature DB >> 28535040 |
Ngoc D B Le, Gulen Yesilbag Tonga, Rubul Mout, Sung-Tae Kim1, Marcos E Wille, Subinoy Rana2, Karen A Dunphy, D Joseph Jerry, Mahdieh Yazdani, Rajesh Ramanathan3, Caren M Rotello, Vincent M Rotello.
Abstract
We report a nanosensor that uses cell lysates to rapidly profile the tumorigenicity of cancer cells. This sensing platform uses host-guest interactions between cucurbit[7]uril and the cationic headgroup of a gold nanoparticle to non-covalently modify the binding of three fluorescent proteins of a multi-channel sensor in situ. This approach doubles the number of output channels to six, providing single-well identification of cell lysates with 100% accuracy. Significantly, this classification could be extended beyond the training set, determining the invasiveness of novel cell lines. The unique fingerprint of these cell lysates required minimal sample quantity (200 ng, ∼1000 cells), making the methodology compatible with microbiopsy technology.Entities:
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Year: 2017 PMID： 28535040 PMCID： PMC5848078 DOI： 10.1021/jacs.7b03657
Source DB: PubMed Journal: J Am Chem Soc ISSN： 0002-7863 Impact factor: 15.419