Literature DB >> 2850697

Production of active human carbonic anhydrase II in E. coli.

C Forsman1, G Behravan, A Osterman, B H Jonsson.   

Abstract

cDNA encoding human carbonic anhydrase II has been isolated and its nucleotide sequence determined. Expression of the isolated carbonic anhydrase gene in Escherichia coli from a plasmid containing the tac promoter yielded an active enzyme at a level of about 1% of total protein.

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Year:  1988        PMID： 2850697     DOI： 10.3891/acta.chem.scand.42b-0314

Source DB:  PubMed          Journal:  Acta Chem Scand B        ISSN： 0302-4369


  16 in total

1.  Carbonic anhydrase is essential for growth of Ralstonia eutropha at ambient CO(2) concentrations.

Authors:  Bernhard Kusian; Dieter Sültemeyer; Botho Bowien
Journal:  J Bacteriol       Date:  2002-09       Impact factor: 3.490

2.  Structure of a monoclinic polymorph of human carbonic anhydrase II with a doubled a axis.

Authors:  Arthur H Robbins; John F Domsic; Mavis Agbandje-McKenna; Robert McKenna
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2010-04-21

3.  Synchrotron Radiation Provides a Plausible Explanation for the Generation of a Free Radical Adduct of Thioxolone in Mutant Carbonic Anhydrase II.

Authors:  Katherine H Sippel; Caroli Genis; Lakshmanan Govindasamy; Mavis Agbandje-McKenna; James J Kiddle; Brian C Tripp; Robert McKenna
Journal:  J Phys Chem Lett       Date:  2010-10-07       Impact factor: 6.475

4.  Coumarinyl-substituted sulfonamides strongly inhibit several human carbonic anhydrase isoforms: solution and crystallographic investigations.

Authors:  Jason Wagner; Balendu Sankara Avvaru; Arthur H Robbins; Andrea Scozzafava; Claudiu T Supuran; Robert McKenna
Journal:  Bioorg Med Chem       Date:  2010-06-15       Impact factor: 3.641

5.  Structural and catalytic effects of proline substitution and surface loop deletion in the extended active site of human carbonic anhydrase II.

Authors:  Christopher D Boone; Valerio Rasi; Chingkuang Tu; Robert McKenna
Journal:  FEBS J       Date:  2015-03-23       Impact factor: 5.542

6.  Expression of Human Carbonic Anhydrase in the Cyanobacterium Synechococcus PCC7942 Creates a High CO(2)-Requiring Phenotype : Evidence for a Central Role for Carboxysomes in the CO(2) Concentrating Mechanism.

Authors:  G D Price; M R Badger
Journal:  Plant Physiol       Date:  1989-10       Impact factor: 8.340

7.  Carbonic anhydrase II deficiency syndrome in a Belgian family is caused by a point mutation at an invariant histidine residue (107 His----Tyr): complete structure of the normal human CA II gene.

Authors:  P J Venta; R J Welty; T M Johnson; W S Sly; R E Tashian
Journal:  Am J Hum Genet       Date:  1991-11       Impact factor: 11.025

8.  Entrapment of carbon dioxide in the active site of carbonic anhydrase II.

Authors:  John F Domsic; Balendu Sankara Avvaru; Chae Un Kim; Sol M Gruner; Mavis Agbandje-McKenna; David N Silverman; Robert McKenna
Journal:  J Biol Chem       Date:  2008-09-02       Impact factor: 5.157

9.  Structural and catalytic characterization of a thermally stable and acid-stable variant of human carbonic anhydrase II containing an engineered disulfide bond.

Authors:  Christopher D Boone; Andrew Habibzadegan; Chingkuang Tu; David N Silverman; Robert McKenna
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2013-07-13

10.  Structural elucidation of the hormonal inhibition mechanism of the bile acid cholate on human carbonic anhydrase II.

Authors:  Christopher D Boone; Chingkuang Tu; Robert McKenna
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2014-05-30
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