Literature DB >> 2850366

Purification and crystallization of a dimeric form of acetylcholinesterase from Torpedo californica subsequent to solubilization with phosphatidylinositol-specific phospholipase C.

J L Sussman1, M Harel, F Frolow, L Varon, L Toker, A H Futerman, I Silman.   

Abstract

A dimeric form of acetylcholinesterase from Torpedo californica was purified to homogeneity by affinity chromatography subsequent to solubilization with a phosphatidylinositol-specific phospholipase C of bacterial origin. Bipyramidal crystals of the enzyme were obtained from solutions in polyethylene glycol 200. The crystals diffract to 2.0 A (1 A = 0.1 nm) resolution. They were found to be orthorhombic, space group P2221, with a = 163.4(+/- 0.2) A, b = 112.1(+/- 0.2) A, c = 81.3(+/- 0.1) A.

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Year:  1988        PMID： 2850366     DOI： 10.1016/0022-2836(88)90213-6

Source DB:  PubMed          Journal:  J Mol Biol        ISSN： 0022-2836            Impact factor:   5.469


  35 in total

Review 1.  Acetylcholinesterase of Schistosoma mansoni--functional correlates. Contributed in honor of Professor Hans Neurath's 90th birthday.

Authors:  R Arnon; I Silman; R Tarrab-Hazdai
Journal:  Protein Sci       Date:  1999-12       Impact factor: 6.725

2.  Specific chemical and structural damage to proteins produced by synchrotron radiation.

Authors:  M Weik; R B Ravelli; G Kryger; S McSweeney; M L Raves; M Harel; P Gros; I Silman; J Kroon; J L Sussman
Journal:  Proc Natl Acad Sci U S A       Date:  2000-01-18       Impact factor: 11.205

3.  Electrostatic steering at acetylcholine binding sites.

Authors:  Robert H Meltzer; Errol Thompson; Kizhake V Soman; Xing-Zhi Song; Jerry O Ebalunode; Theodore G Wensel; James M Briggs; Steen E Pedersen
Journal:  Biophys J       Date:  2006-06-02       Impact factor: 4.033

4.  Structural insights into substrate traffic and inhibition in acetylcholinesterase.

Authors:  Jacques-Philippe Colletier; Didier Fournier; Harry M Greenblatt; Jure Stojan; Joel L Sussman; Giuseppe Zaccai; Israel Silman; Martin Weik
Journal:  EMBO J       Date:  2006-06-08       Impact factor: 11.598

5.  Shoot-and-Trap: use of specific x-ray damage to study structural protein dynamics by temperature-controlled cryo-crystallography.

Authors:  Jacques-Philippe Colletier; Dominique Bourgeois; Benoît Sanson; Didier Fournier; Joel L Sussman; Israel Silman; Martin Weik
Journal:  Proc Natl Acad Sci U S A       Date:  2008-08-13       Impact factor: 11.205

6.  A mechanism-based 3D-QSAR approach for classification and prediction of acetylcholinesterase inhibitory potency of organophosphate and carbamate analogs.

Authors:  Sehan Lee; Mace G Barron
Journal:  J Comput Aided Mol Des       Date:  2016-04-07       Impact factor: 3.686

7.  In vitro detoxification of cyclosarin in human blood pre-incubated ex vivo with recombinant serum paraoxonases.

Authors:  Yacov Ashani; Moshe Goldsmith; Haim Leader; Israel Silman; Joel L Sussman; Dan S Tawfik
Journal:  Toxicol Lett       Date:  2011-07-23       Impact factor: 4.372

8.  Soluble monomeric acetylcholinesterase from mouse: expression, purification, and crystallization in complex with fasciculin.

Authors:  P Marchot; R B Ravelli; M L Raves; Y Bourne; D C Vellom; J Kanter; S Camp; J L Sussman; P Taylor
Journal:  Protein Sci       Date:  1996-04       Impact factor: 6.725

9.  The active site and partial sequence of cobra venom acetylcholinesterase.

Authors:  C Weise; H J Kreienkamp; R Raba; A Aaviksaar; F Hucho
Journal:  J Protein Chem       Date:  1990-02

10.  Design, expression and characterization of mutants of fasciculin optimized for interaction with its target, acetylcholinesterase.

Authors:  Oz Sharabi; Yoav Peleg; Efrat Mashiach; Eyal Vardy; Yacov Ashani; Israel Silman; Joel L Sussman; Julia M Shifman
Journal:  Protein Eng Des Sel       Date:  2009-07-30       Impact factor: 1.650
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