Thamara C Peixoto1, Egberto G Moura1, Elaine de Oliveira1, Patrícia N Soares1, Deysla S Guarda1, Dayse N Bernardino1, Xu Xue Ai1, Vanessa da S T Rodrigues1, Gabriela Rodrigues de Souza2, Antonio Jorge Ribeiro da Silva2, Mariana S Figueiredo1, Alex C Manhães3, Patrícia C Lisboa4. 1. Laboratory of Endocrine Physiology, Departamento de Ciências Fisiológicas, 5o andar, Instituto de Biologia, Universidade do Estado do Rio de Janeiro, Av. 28 de Setembro, 87, Rio de Janeiro, RJ, 20551-031, Brazil. 2. Department of Program of Natural Products, Phytochemical Analysis Laboratory, Institute for Natural Products Research, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, Brazil. 3. Laboratory of Neurophysiology, Departamento de Ciências Fisiológicas, 5o andar, Instituto de Biologia, Universidade do Estado do Rio de Janeiro, Av. 28 de Setembro, 87, Rio de Janeiro, RJ, 20551-031, Brazil. 4. Laboratory of Endocrine Physiology, Departamento de Ciências Fisiológicas, 5o andar, Instituto de Biologia, Universidade do Estado do Rio de Janeiro, Av. 28 de Setembro, 87, Rio de Janeiro, RJ, 20551-031, Brazil. pclisboa@uerj.br.
Abstract
PURPOSE: Obese individuals have higher production of reactive oxygen species, which leads to oxidative damage. We hypothesize that cranberry extract (CE) can improve this dysfunction in HFD-induced obesity in rats since it has an important antioxidant activity. Here, we evaluated the effects of CE in food intake, adiposity, biochemical and hormonal parameters, lipogenic and adipogenic factors, hepatic morphology and oxidative balance in a HFD model. METHODS: At postnatal day 120 (PN120), male Wistar rats were assigned into two groups: (1) SD (n = 36) fed with a standard diet and (2) HFD (n = 36), fed with a diet containing 44.5% (35.2% from lard) energy from fat. At PN150, 12 animals from SD and HFD groups were killed while the others were subdivided into four groups (n = 12/group): animals that received 200 mg/kg cranberry extract (SD CE, HFD CE) gavage/daily/30 days or water (SD, HFD). At PN180, animals were killed. RESULTS: HFD group showed higher body mass and visceral fat, hypercorticosteronemia, higher liver glucocorticoid sensitivity, cholesterol and triglyceride contents and microsteatosis. Also, HFD group had higher lipid peroxidation (plasma and tissues) and higher protein carbonylation (liver and adipose tissue) compared to SD group. HFD CE group showed lower body mass gain, hypotrygliceridemia, hypocorticosteronemia, and lower hepatic cholesterol and fatty acid synthase contents. HFD CE group displayed lower lipid peroxidation, protein carbonylation (liver and adipose tissue) and accumulation of liver fat compared to HFD group. CONCLUSION: Although adiposity was not completely reversed, cranberry extract improved the metabolic profile and reduced oxidative damage and steatosis in HFD-fed rats, which suggests that it can help manage obesity-related disorders.
PURPOSE:Obese individuals have higher production of reactive oxygen species, which leads to oxidative damage. We hypothesize that cranberry extract (CE) can improve this dysfunction in HFD-induced obesity in rats since it has an important antioxidant activity. Here, we evaluated the effects of CE in food intake, adiposity, biochemical and hormonal parameters, lipogenic and adipogenic factors, hepatic morphology and oxidative balance in a HFD model. METHODS: At postnatal day 120 (PN120), male Wistar rats were assigned into two groups: (1) SD (n = 36) fed with a standard diet and (2) HFD (n = 36), fed with a diet containing 44.5% (35.2% from lard) energy from fat. At PN150, 12 animals from SD and HFD groups were killed while the others were subdivided into four groups (n = 12/group): animals that received 200 mg/kg cranberry extract (SD CE, HFD CE) gavage/daily/30 days or water (SD, HFD). At PN180, animals were killed. RESULTS: HFD group showed higher body mass and visceral fat, hypercorticosteronemia, higher liver glucocorticoid sensitivity, cholesterol and triglyceride contents and microsteatosis. Also, HFD group had higher lipid peroxidation (plasma and tissues) and higher protein carbonylation (liver and adipose tissue) compared to SD group. HFD CE group showed lower body mass gain, hypotrygliceridemia, hypocorticosteronemia, and lower hepatic cholesterol and fatty acid synthase contents. HFD CE group displayed lower lipid peroxidation, protein carbonylation (liver and adipose tissue) and accumulation of liver fat compared to HFD group. CONCLUSION: Although adiposity was not completely reversed, cranberry extract improved the metabolic profile and reduced oxidative damage and steatosis in HFD-fed rats, which suggests that it can help manage obesity-related disorders.
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