| Literature DB >> 28495876 |
Peter J Thul1, Lovisa Åkesson1, Mikaela Wiking1, Diana Mahdessian1, Aikaterini Geladaki2,3, Hammou Ait Blal1, Tove Alm1, Anna Asplund4, Lars Björk1, Lisa M Breckels2,5, Anna Bäckström1, Frida Danielsson1, Linn Fagerberg1, Jenny Fall1, Laurent Gatto2,5, Christian Gnann1, Sophia Hober6, Martin Hjelmare1, Fredric Johansson1, Sunjae Lee1, Cecilia Lindskog4, Jan Mulder7, Claire M Mulvey2, Peter Nilsson1, Per Oksvold1, Johan Rockberg6, Rutger Schutten1, Jochen M Schwenk1, Åsa Sivertsson1, Evelina Sjöstedt4, Marie Skogs1, Charlotte Stadler1, Devin P Sullivan1, Hanna Tegel6, Casper Winsnes1, Cheng Zhang1, Martin Zwahlen1, Adil Mardinoglu1, Fredrik Pontén4, Kalle von Feilitzen1, Kathryn S Lilley2, Mathias Uhlén8, Emma Lundberg8.
Abstract
Resolving the spatial distribution of the human proteome at a subcellular level can greatly increase our understanding of human biology and disease. Here we present a comprehensive image-based map of subcellular protein distribution, the Cell Atlas, built by integrating transcriptomics and antibody-based immunofluorescence microscopy with validation by mass spectrometry. Mapping the in situ localization of 12,003 human proteins at a single-cell level to 30 subcellular structures enabled the definition of the proteomes of 13 major organelles. Exploration of the proteomes revealed single-cell variations in abundance or spatial distribution and localization of about half of the proteins to multiple compartments. This subcellular map can be used to refine existing protein-protein interaction networks and provides an important resource to deconvolute the highly complex architecture of the human cell.Entities:
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Year: 2017 PMID： 28495876 DOI： 10.1126/science.aal3321
Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728