Literature DB >> 2848016

Allele replacement in Escherichia coli by use of a selectable marker for resistance to spectinomycin: replacement of the lexA gene.

S A Hill1, J W Little.   

Abstract

We replaced the Escherichia coli lexA gene by a segment of DNA coding for resistance to spectinomycin and streptomycin. The use of this segment expands the range of selectable markers usable for allele replacement. The availability of this null lexA mutation will facilitate genetic analysis of lexA and the SOS regulon.

Entities:  

Mesh：

Substances：

Year:  1988        PMID： 2848016      PMCID： PMC211701          DOI： 10.1128/jb.170.12.5913-5915.1988

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  18 in total

1.  The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library.

Authors:  Y Kohara; K Akiyama; K Isono
Journal:  Cell       Date:  1987-07-31       Impact factor: 41.582

2.  Site-directed insertion and deletion mutagenesis with cloned fragments in Escherichia coli.

Authors:  S C Winans; S J Elledge; J H Krueger; G C Walker
Journal:  J Bacteriol       Date:  1985-03       Impact factor: 3.490

3.  Nucleotide sequence of the lexA gene of Escherichia coli K-12.

Authors:  B E Markham; J W Little; D W Mount
Journal:  Nucleic Acids Res       Date:  1981-08-25       Impact factor: 16.971

Review 4.  Mutagenesis and inducible responses to deoxyribonucleic acid damage in Escherichia coli.

Authors:  G C Walker
Journal:  Microbiol Rev       Date:  1984-03

5.  Escherichia coli RecBC pseudorevertants lacking chi recombinational hotspot activity.

Authors:  D W Schultz; A F Taylor; G R Smith
Journal:  J Bacteriol       Date:  1983-08       Impact factor: 3.490

6.  New M13 vectors for cloning.

Authors:  J Messing
Journal:  Methods Enzymol       Date:  1983       Impact factor: 1.600

7.  The DNA sequences encoding plsB and dgk loci of Escherichia coli.

Authors:  V A Lightner; R M Bell; P Modrich
Journal:  J Biol Chem       Date:  1983-09-25       Impact factor: 5.157

Review 8.  The SOS regulatory system of Escherichia coli.

Authors:  J W Little; D W Mount
Journal:  Cell       Date:  1982-05       Impact factor: 41.582

9.  Sensitivity and Resistance to Spectinomycin in Escherichia coli.

Authors:  P Anderson
Journal:  J Bacteriol       Date:  1969-11       Impact factor: 3.490

10.  Isolation and characterization of noncleavable (Ind-) mutants of the LexA repressor of Escherichia coli K-12.

Authors:  L L Lin; J W Little
Journal:  J Bacteriol       Date:  1988-05       Impact factor: 3.490
View more
  7 in total

1.  A phenotype for enigmatic DNA polymerase II: a pivotal role for pol II in replication restart in UV-irradiated Escherichia coli.

Authors:  S Rangarajan; R Woodgate; M F Goodman
Journal:  Proc Natl Acad Sci U S A       Date:  1999-08-03       Impact factor: 11.205

2.  The genetic requirements for UmuDC-mediated cold sensitivity are distinct from those for SOS mutagenesis.

Authors:  T Opperman; S Murli; G C Walker
Journal:  J Bacteriol       Date:  1996-08       Impact factor: 3.490

3.  One-step cloning system for isolation of bacterial lexA-like genes.

Authors:  S Calero; X Garriga; J Barbé
Journal:  J Bacteriol       Date:  1991-11       Impact factor: 3.490

4.  Regulation of the SOS response in Bacillus subtilis: evidence for a LexA repressor homolog.

Authors:  M F Wojciechowski; K R Peterson; P E Love
Journal:  J Bacteriol       Date:  1991-10       Impact factor: 3.490

5.  Nucleotide sequence analysis and comparison of the lexA genes from Salmonella typhimurium, Erwinia carotovora, Pseudomonas aeruginosa and Pseudomonas putida.

Authors:  X Garriga; S Calero; J Barbé
Journal:  Mol Gen Genet       Date:  1992-12

6.  Phenotypes of lexA mutations in Salmonella enterica: evidence for a lethal lexA null phenotype due to the Fels-2 prophage.

Authors:  Kim Bunny; Jing Liu; John Roth
Journal:  J Bacteriol       Date:  2002-11       Impact factor: 3.490

7.  Use of adaptive laboratory evolution to discover key mutations enabling rapid growth of Escherichia coli K-12 MG1655 on glucose minimal medium.

Authors:  Ryan A LaCroix; Troy E Sandberg; Edward J O'Brien; Jose Utrilla; Ali Ebrahim; Gabriela I Guzman; Richard Szubin; Bernhard O Palsson; Adam M Feist
Journal:  Appl Environ Microbiol       Date:  2014-10-10       Impact factor: 4.792
  7 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.