Literature DB >> 28477126

Imaging of Fluorescently Tagged ATM Kinase at the Sites of DNA Double Strand Breaks.

Anthony J Davis1, Shih-Ya Wang1, David J Chen1, Benjamin P C Chen2.   

Abstract

The combination of live cell imaging and laser micro-irradiation is an important technique to investigate the recruitment and kinetics of DNA repair molecules to DNA damage sites. In this chapter, we describe the detailed methods to study the dynamics of fluorescently tagged ATM protein kinase at laser-induced DNA double strand break (DSB) sites. The same protocol can be applied to analyze the recruitment and kinetics of other potential or known DNA repair proteins to DSB sites.

Entities:  

Keywords:  ATM kinase; DNA double strand breaks; Green fluorescent protein; Laser micro-irradiation

Mesh：

Substances：

Year:  2017        PMID： 28477126      PMCID： PMC6085091          DOI： 10.1007/978-1-4939-6955-5_20

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  6 in total

1.  Dynamics of the PI3K-like protein kinase members ATM and DNA-PKcs at DNA double strand breaks.

Authors:  Anthony J Davis; Sairei So; David J Chen
Journal:  Cell Cycle       Date:  2010-07-01       Impact factor: 4.534

2.  Autophosphorylation at serine 1981 stabilizes ATM at DNA damage sites.

Authors:  Sairei So; Anthony J Davis; David J Chen
Journal:  J Cell Biol       Date:  2009-12-21       Impact factor: 10.539

3.  Establishment and characterization of a permanent pSV ori--transformed ataxia-telangiectasia cell line.

Authors:  J P Murnane; L F Fuller; R B Painter
Journal:  Exp Cell Res       Date:  1985-05       Impact factor: 3.905

4.  DNA damage activates ATM through intermolecular autophosphorylation and dimer dissociation.

Authors:  Christopher J Bakkenist; Michael B Kastan
Journal:  Nature       Date:  2003-01-30       Impact factor: 49.962

5.  Ku recruits XLF to DNA double-strand breaks.

Authors:  Ken-ichi Yano; Keiko Morotomi-Yano; Shih-Ya Wang; Naoya Uematsu; Kyung-Jong Lee; Aroumougame Asaithamby; Eric Weterings; David J Chen
Journal:  EMBO Rep       Date:  2007-12-07       Impact factor: 8.807

6.  Autophosphorylation of DNA-PKCS regulates its dynamics at DNA double-strand breaks.

Authors:  Naoya Uematsu; Eric Weterings; Ken-ichi Yano; Keiko Morotomi-Yano; Burkhard Jakob; Gisela Taucher-Scholz; Pierre-Olivier Mari; Dik C van Gent; Benjamin P C Chen; David J Chen
Journal:  J Cell Biol       Date:  2007-04-16       Impact factor: 10.539
  6 in total

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