| Literature DB >> 28475900 |
Yonit Lavin1, Soma Kobayashi1, Andrew Leader1, El-Ad David Amir2, Naama Elefant3, Camille Bigenwald1, Romain Remark1, Robert Sweeney4, Christian D Becker5, Jacob H Levine6, Klaus Meinhof5, Andrew Chow1, Seunghee Kim-Shulze2, Andrea Wolf7, Chiara Medaglia3, Hanjie Li3, Julie A Rytlewski8, Ryan O Emerson8, Alexander Solovyov9, Benjamin D Greenbaum9, Catherine Sanders8, Marissa Vignali8, Mary Beth Beasley10, Raja Flores7, Sacha Gnjatic11, Dana Pe'er6, Adeeb Rahman12, Ido Amit3, Miriam Merad13.
Abstract
To guide the design of immunotherapy strategies for patients with early stage lung tumors, we developed a multiscale immune profiling strategy to map the immune landscape of early lung adenocarcinoma lesions to search for tumor-driven immune changes. Utilizing a barcoding method that allows a simultaneous single-cell analysis of the tumor, non-involved lung, and blood cells, we provide a detailed immune cell atlas of early lung tumors. We show that stage I lung adenocarcinoma lesions already harbor significantly altered T cell and NK cell compartments. Moreover, we identified changes in tumor-infiltrating myeloid cell (TIM) subsets that likely compromise anti-tumor T cell immunity. Paired single-cell analyses thus offer valuable knowledge of tumor-driven immune changes, providing a powerful tool for the rational design of immune therapies. VIDEO ABSTRACT.Entities:
Keywords: CD141+ DC; CD1c+ DC; NK Cell; T Cell; TIM; TLS; human non-small cell lung cancer (NSCLC); immune cell atlas; lung adenocarcinoma; tumor macrophage
Mesh：
Year: 2017 PMID： 28475900 PMCID： PMC5737939 DOI： 10.1016/j.cell.2017.04.014
Source DB: PubMed Journal: Cell ISSN： 0092-8674 Impact factor: 41.582