Literature DB >> 2847588

Determination of glucose-6-phosphatase activity using the glucose dehydrogenase-coupled reaction.

M Alegre1, C J Ciudad, C Fillat, J J Guinovart.   

Abstract

We present a method to determine glucose 6-phosphate activity. This assay measures the rate of glucose released in the glucose-6-phosphatase reaction. The glucose is oxidized to beta-D-gluconolactone by glucose dehydrogenase in a coupled reaction that uses NAD(P)+. The determination is rapid, reproducible, and does not require withdrawal, precipitation, centrifugation, or neutralization steps. This method provides a simple resolution to the problem of the nonspecific appearance of Pi, which is especially important in studies of regulation of glucose-6-phosphatase performed in the presence of ATP.

Entities:  

Mesh：

Substances：

Year:  1988        PMID： 2847588     DOI： 10.1016/0003-2697(88)90176-5

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  40 in total

1.  Long-term feeding a plant-based diet devoid of marine ingredients strongly affects certain key metabolic enzymes in the rainbow trout liver.

Authors:  Vincent Véron; Stéphane Panserat; Richard Le Boucher; Laurent Labbé; Edwige Quillet; Mathilde Dupont-Nivet; Françoise Médale
Journal:  Fish Physiol Biochem       Date:  2016-01-08       Impact factor: 2.794

2.  Exposure of cells to a cell number-counting factor decreases the activity of glucose-6-phosphatase to decrease intracellular glucose levels in Dictyostelium discoideum.

Authors:  Wonhee Jang; Richard H Gomer
Journal:  Eukaryot Cell       Date:  2005-01

3.  An assay for glucose 6-phosphatase based on the formation of glucose.

Authors:  C Bublitz
Journal:  Mol Cell Biochem       Date:  1991-12-11       Impact factor: 3.396

4.  The control of hepatic glycogen metabolism in an in vitro model of sepsis.

Authors:  Jennifer Wallington; Jian Ning; Michael Alan Titheradge
Journal:  Mol Cell Biochem       Date:  2007-10-13       Impact factor: 3.396

5.  Effects of dietary amylose/amylopectin ratio on growth performance, feed utilization, digestive enzymes, and postprandial metabolic responses in juvenile obscure puffer Takifugu obscurus.

Authors:  Xiang-he Liu; Chao-xia Ye; Ji-dan Ye; Bi-duan Shen; Chun-yan Wang; An-li Wang
Journal:  Fish Physiol Biochem       Date:  2014-04-08       Impact factor: 2.794

6.  PAHSAs enhance hepatic and systemic insulin sensitivity through direct and indirect mechanisms.

Authors:  Peng Zhou; Anna Santoro; Odile D Peroni; Andrew T Nelson; Alan Saghatelian; Dionicio Siegel; Barbara B Kahn
Journal:  J Clin Invest       Date:  2019-10-01       Impact factor: 14.808

7.  Aged ginseng (Panax ginseng Meyer) reduces blood glucose levels and improves lipid metabolism in high fat diet-fed mice.

Authors:  Soo Im Chung; Su Jin Nam; Mingze Xu; Mi Young Kang; Sang Chul Lee
Journal:  Food Sci Biotechnol       Date:  2016-02-29       Impact factor: 2.391

8.  ChREBP regulates fructose-induced glucose production independently of insulin signaling.

Authors:  Mi-Sung Kim; Sarah A Krawczyk; Ludivine Doridot; Alan J Fowler; Jennifer X Wang; Sunia A Trauger; Hye-Lim Noh; Hee Joon Kang; John K Meissen; Matthew Blatnik; Jason K Kim; Michelle Lai; Mark A Herman
Journal:  J Clin Invest       Date:  2016-09-26       Impact factor: 14.808

9.  Modulatory Effect of Rice Bran and Phytic Acid on Glucose Metabolism in High Fat-Fed C57BL/6N Mice.

Authors:  Soo Mi Kim; Catherine W Rico; Sang Chul Lee; Mi Young Kang
Journal:  J Clin Biochem Nutr       Date:  2010-04-10       Impact factor: 3.114

10.  Enzymatic capacities of metabolic fuel use in cuttlefish (Sepia officinalis) and responses to food deprivation: insight into the metabolic organization and starvation survival strategy of cephalopods.

Authors:  Ben Speers-Roesch; Neal I Callaghan; Tyson J MacCormack; Simon G Lamarre; Antonio V Sykes; William R Driedzic
Journal:  J Comp Physiol B       Date:  2016-04-30       Impact factor: 2.200
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.