Literature DB >> 1664041

An assay for glucose 6-phosphatase based on the formation of glucose.

C Bublitz1.   

Abstract

A rapid, convenient method for the assay of glucose 6-phosphatase dependent on the removal of radioactive substrate from radioactive product by Dowex 2 fluoride is described. The enzymatic reaction is stopped by the addition of an ethanolic slurry of the resin. After the tubes are shaken, the radioactivity of glucose in the clarified supernatant layer is measured. The release of glucose is directly proportional to time and enzyme concentration. Detergents do not interfere with the method.

Entities:  

Mesh：

Substances：

Year:  1991        PMID： 1664041     DOI： 10.1007/bf00233118

Source DB:  PubMed          Journal:  Mol Cell Biochem        ISSN： 0300-8177            Impact factor:   3.396


  10 in total

1.  A CHEMICAL METHOD FOR THE QUANTITATIVE DETERMINATION OF ADENOSINE 3',5'-PHOSPHATE.

Authors:  L S BRADHAM; D W WOOLLEY
Journal:  Biochim Biophys Acta       Date:  1964-12-09

2.  A batch procedure for the assay of cyclic nucleotide phosphodiesterase.

Authors:  C Bublitz
Journal:  Anal Biochem       Date:  1978-07-15       Impact factor: 3.365

3.  The topology of phosphogluconate dehydrogenases in rat liver microsomes.

Authors:  C Bublitz; C A Lawler; S Steavenson
Journal:  Arch Biochem Biophys       Date:  1987-11-15       Impact factor: 4.013

4.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

Review 5.  Glucose-6-phosphatase: two concepts of membrane-function relationship.

Authors:  K A Sukalski; R C Nordlie
Journal:  Adv Enzymol Relat Areas Mol Biol       Date:  1989

6.  Determination of glucose-6-phosphatase activity using the glucose dehydrogenase-coupled reaction.

Authors:  M Alegre; C J Ciudad; C Fillat; J J Guinovart
Journal:  Anal Biochem       Date:  1988-08-15       Impact factor: 3.365

7.  Kinetics of the glucose 6-phosphate-glucose exchange activity and glucose inhibition of glucose 6-phosphatase of intact and disrupted rat liver microsomes.

Authors:  W J Arion; B K Wallin
Journal:  J Biol Chem       Date:  1973-04-10       Impact factor: 5.157

8.  Distribution of constitutive enzymes and phospholipids in microsomal membranes of rat liver.

Authors:  O Nilsson; G Dallner
Journal:  FEBS Lett       Date:  1975-10-15       Impact factor: 4.124

9.  A continuous spectrophotometric assay for the enzymatic marker glucose 6-phosphatase.

Authors:  M Stio; P Vanni; G Pinzauti
Journal:  Anal Biochem       Date:  1988-10       Impact factor: 3.365

10.  DIFFERENTIATION OF ENDOPLASMIC RETICULUM IN HEPATOCYTES : II. Glucose-6-Phosphatase in Rough Microsomes.

Authors:  A Leskes; P Siekevitz; G E Palade
Journal:  J Cell Biol       Date:  1971-05-01       Impact factor: 10.539
  10 in total
  1 in total

1.  Regulation of microsomal triglyceride transfer protein by apolipoprotein A-IV in newborn swine intestinal epithelial cells.

Authors:  Ying Yao; Song Lu; Yue Huang; Casey C Beeman-Black; Rena Lu; Xiaoyue Pan; M Mahmood Hussain; Dennis D Black
Journal:  Am J Physiol Gastrointest Liver Physiol       Date:  2010-12-02       Impact factor: 4.052
  1 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.