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Literature DB >> 2847174

Site of covalent attachment of alpha-scorpion toxin derivatives in domain I of the sodium channel alpha subunit.

Abstract

Purified and reconstituted sodium channels from rat brain have been photoaffinity labeled with a photoactivable derivative of the alpha-scorpion toxin V from Leiurus quinquestriatus (LqTx). A battery of sequence-specific antibodies has been used to determine which of the peptides produced by chemical and enzymatic cleavage of the photolabeled sodium-channel alpha subunit contain covalently attached LqTx. Nearly all of the covalently attached LqTx is found within homologous domain I. Two site-directed antisera, which recognize residues 317 to 335 and residues 382 to 400, respectively, specifically immunoprecipitate a 14-kDa peptide produced by CNBr digestion to which LqTx is covalently attached. It is proposed that a portion of the receptor site for alpha-scorpion toxins is formed by peptide segment(s) between amino acid residues 335 and 378 which is located in an extracellular loop between transmembrane helices S5 and S6 of homologous domain I of the sodium channel alpha subunit.

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Year: 1988 PMID： 2847174 PMCID： PMC282537 DOI： 10.1073/pnas.85.22.8742

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

36 in total

1. Activation of the action potential Na+ ionophore by neurotoxins. An allosteric model.

Authors: W A Catterall
Journal: J Biol Chem Date: 1977-12-10 Impact factor: 5.157

Review 2. Scorpion toxins: chemistry and mode of action.

Authors: H Rochat; P Bernard; F Couraud
Journal: Adv Cytopharmacol Date: 1979

3. A discontinuous electrophoretic system for separating peptides on polyacrylamide gels.

Authors: J Kyte; H Rodriguez
Journal: Anal Biochem Date: 1983-09 Impact factor: 3.365

4. Alpha-scorpion neurotoxin derivatives suitable as potential markers of sodium channels. Preparation and characterization.

Authors: H Darbon; E Jover; F Couraud; H Rochat
Journal: Int J Pept Protein Res Date: 1983-08

5. The three-dimensional structure of scorpion neurotoxins.

Authors: J C Fontecilla-Camps; R J Almassy; F L Suddath; C E Bugg
Journal: Toxicon Date: 1982 Impact factor: 3.033

6. Covalent labeling of protein components of the sodium channel with a photoactivable derivative of scorpion toxin.

Authors: D A Beneski; W A Catterall
Journal: Proc Natl Acad Sci U S A Date: 1980-01 Impact factor: 11.205

Review 7. Neurotoxins that act on voltage-sensitive sodium channels in excitable membranes.

Authors: W A Catterall
Journal: Annu Rev Pharmacol Toxicol Date: 1980 Impact factor: 13.820

8. Two types of scorpion receptor sites, one related to the activation, the other to the inactivation of the action potential sodium channel.

Authors: F Couraud; E Jover; J M Dubois; H Rochat
Journal: Toxicon Date: 1982 Impact factor: 3.033

9. Toxin T4(6) from Ptychodiscus brevis (formerly Gymnodinium breve) enhances activation of voltage-sensitive sodium channels by veratridine.

Authors: W A Catterall; M Risk
Journal: Mol Pharmacol Date: 1981-03 Impact factor: 4.436

10. Binding of scorpion toxin to receptor sites associated with sodium channels in frog muscle. Correlation of voltage-dependent binding with activation.

Authors: W A Catterall
Journal: J Gen Physiol Date: 1979-09 Impact factor: 4.086

36 in total

8. Photoaffinity labeling of the receptor site for alpha-scorpion toxins on purified and reconstituted sodium channels by a new toxin derivative.

Authors: F J Tejedor; W A Catterall
Journal: Cell Mol Neurobiol Date: 1990-06 Impact factor: 5.046

9. Molecular determinants of beta 1 subunit-induced gating modulation in voltage-dependent Na+ channels.

Authors: N Makita; P B Bennett; A L George
Journal: J Neurosci Date: 1996-11-15 Impact factor: 6.167

10. Modulation of Na+ channel inactivation by the beta 1 subunit: a deletion analysis.

Authors: C Chen; S C Cannon
Journal: Pflugers Arch Date: 1995-12 Impact factor: 3.657

Site of covalent attachment of alpha-scorpion toxin derivatives in domain I of the sodium channel alpha subunit.

1. Activation of the action potential Na+ ionophore by neurotoxins. An allosteric model.

Review 2. Scorpion toxins: chemistry and mode of action.

3. A discontinuous electrophoretic system for separating peptides on polyacrylamide gels.

4. Alpha-scorpion neurotoxin derivatives suitable as potential markers of sodium channels. Preparation and characterization.

5. The three-dimensional structure of scorpion neurotoxins.

6. Covalent labeling of protein components of the sodium channel with a photoactivable derivative of scorpion toxin.

Review 7. Neurotoxins that act on voltage-sensitive sodium channels in excitable membranes.

8. Two types of scorpion receptor sites, one related to the activation, the other to the inactivation of the action potential sodium channel.

9. Toxin T4(6) from Ptychodiscus brevis (formerly Gymnodinium breve) enhances activation of voltage-sensitive sodium channels by veratridine.

10. Binding of scorpion toxin to receptor sites associated with sodium channels in frog muscle. Correlation of voltage-dependent binding with activation.

1. The outermost lysine in the S4 of domain III contributes little to the gating charge in sodium channels.

Review 2. Molecular mechanism of scorpion neurotoxins acting on sodium channels: insight into their diverse selectivity.

3. Structure and function of the voltage sensor of sodium channels probed by a beta-scorpion toxin.

4. The quantal gating charge of sodium channel inactivation.

Review 5. Voltage-gated sodium channel modulation by scorpion alpha-toxins.

Review 6. Site-3 toxins and cardiac sodium channels.

Review 7. Molecular basis of drug interaction with L-type Ca2+ channels.

8. Photoaffinity labeling of the receptor site for alpha-scorpion toxins on purified and reconstituted sodium channels by a new toxin derivative.

9. Molecular determinants of beta 1 subunit-induced gating modulation in voltage-dependent Na+ channels.

10. Modulation of Na+ channel inactivation by the beta 1 subunit: a deletion analysis.