| Literature DB >> 28469032 |
Nunya Chotiwan1,2, Connie D Brewster1, Tereza Magalhaes2,3, James Weger-Lucarelli1,2, Nisha K Duggal4, Claudia Rückert1,2, Chilinh Nguyen1,2, Selene M Garcia Luna1,2, Joseph R Fauver1,2, Barb Andre1, Meg Gray1,2, William C Black1,2, Rebekah C Kading1,2, Gregory D Ebel1,2, Guillermina Kuan5, Angel Balmaseda6, Thomas Jaenisch7,8, Ernesto T A Marques3,9, Aaron C Brault4, Eva Harris10, Brian D Foy1,2, Sandra L Quackenbush1, Rushika Perera1,2, Joel Rovnak11.
Abstract
Understanding the dynamics of Zika virus transmission and formulating rational strategies for its control require precise diagnostic tools that are also appropriate for resource-poor environments. We have developed a rapid and sensitive loop-mediated isothermal amplification (LAMP) assay that distinguishes Zika viruses of Asian and African lineages. The assay does not detect chikungunya virus or flaviviruses such as dengue, yellow fever, or West Nile viruses. The assay conditions allowed direct detection of Zika virus RNA in cultured infected cells; in mosquitoes; in virus-spiked samples of human blood, plasma, saliva, urine, and semen; and in infected patient serum, plasma, and semen samples without the need for RNA isolation or reverse transcription. The assay offers rapid, specific, sensitive, and inexpensive detection of the Asian-lineage Zika virus strain that is currently circulating in the Western hemisphere, and can also detect the African-lineage Zika virus strain using separate, specific primers.Entities:
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Year: 2017 PMID： 28469032 PMCID： PMC5654541 DOI： 10.1126/scitranslmed.aag0538
Source DB: PubMed Journal: Sci Transl Med ISSN： 1946-6234 Impact factor: 17.956