Literature DB >> 2846538

Synthesis and assembly of a functionally active recombinant platelet-derived growth factor AB heterodimer.

A Ostman1, L Rall, A Hammacher, M A Wormstead, D Coit, P Valenzuela, C Betsholtz, B Westermark, C H Heldin.   

Abstract

A Chinese hamster ovary cell line that stably expresses transfected human platelet-derived growth factor (PDGF) A and B chain precursors was established. All three dimeric combinations of PDGF chains were produced by this cell line; their biosynthesis, assembly, and processing were followed by pulse-chase analyses. PDGF-AA, PDGF-AB, and PDGF-BB were processed to Mr values of about 30,000 and were accumulated in these forms in the medium. In addition, PDGF-BB was further processed to a 24-kDa component, which remained cell-associated. The major secreted component was PDGF-AB, which was purified and shown to have structural and functional characteristics indistinguishable from PDGF-AB purified from human platelets.

Entities:  

Mesh：

Substances：

Year:  1988        PMID： 2846538

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  15 in total

Review 1.  Cytokines in asthma.

Authors:  K F Chung; P J Barnes
Journal:  Thorax       Date:  1999-09       Impact factor: 9.139

2.  Purification and analysis of proteinase-resistant mutants of recombinant platelet-derived growth factor-BB exhibiting improved biological activity.

Authors:  A L Cook; P M Kirwin; S Craig; L J Bawden; D R Green; M J Price; S J Richardson; A Fallon; A H Drummond; R M Edwards
Journal:  Biochem J       Date:  1992-01-01       Impact factor: 3.857

Review 3.  Platelet-derived growth factor: mechanism of action and possible in vivo function.

Authors:  C H Heldin; B Westermark
Journal:  Cell Regul       Date:  1990-07

4.  Differential effects of the various isoforms of platelet-derived growth factor on chemotaxis of fibroblasts, monocytes, and granulocytes.

Authors:  A Siegbahn; A Hammacher; B Westermark; C H Heldin
Journal:  J Clin Invest       Date:  1990-03       Impact factor: 14.808

5.  Proteolytic processing regulates receptor specificity and activity of VEGF-C.

Authors:  V Joukov; T Sorsa; V Kumar; M Jeltsch; L Claesson-Welsh; Y Cao; O Saksela; N Kalkkinen; K Alitalo
Journal:  EMBO J       Date:  1997-07-01       Impact factor: 11.598

6.  The A-type receptor for platelet-derived growth factor mediates protein tyrosine phosphorylation, receptor transmodulation and a mitogenic response.

Authors:  A Hammacher; M Nistér; C H Heldin
Journal:  Biochem J       Date:  1989-11-15       Impact factor: 3.857

7.  PDGF mediates cardiac microvascular communication.

Authors:  J M Edelberg; W C Aird; W Wu; H Rayburn; W S Mamuya; M Mercola; R D Rosenberg
Journal:  J Clin Invest       Date:  1998-08-15       Impact factor: 14.808

8.  Reversion of autocrine transformation by a dominant negative platelet-derived growth factor mutant.

Authors:  F S Vassbotn; M Andersson; B Westermark; C H Heldin; A Ostman
Journal:  Mol Cell Biol       Date:  1993-07       Impact factor: 4.272

9.  Identification of a cell retention signal in the B-chain of platelet-derived growth factor and in the long splice version of the A-chain.

Authors:  A Ostman; M Andersson; C Betsholtz; B Westermark; C H Heldin
Journal:  Cell Regul       Date:  1991-07

10.  Platelet-derived growth factor (PDGF) in oncogenesis: development of a vascular connective tissue stroma in xenotransplanted human melanoma producing PDGF-BB.

Authors:  K Forsberg; I Valyi-Nagy; C H Heldin; M Herlyn; B Westermark
Journal:  Proc Natl Acad Sci U S A       Date:  1993-01-15       Impact factor: 11.205
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.