Literature DB >> 2846239

Ageing of fixed cytological preparations produces degradation of chromosomal DNA.

R Mezzanotte1, R Vanni, O Flore, L Ferrucci, A T Sumner.   

Abstract

When fixed chromosome preparations were allowed to age for 1-72 h, they became progressively more susceptible to digestion by exonuclease III and by S1 nuclease. Analysis of DNA from these aged preparations on agarose gels showed that the molecular weight of the DNA decreased as ageing progressed. We conclude that DNA in fixed chromosome preparations becomes progressively degraded as the preparations age.

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Year:  1988        PMID： 2846239     DOI： 10.1159/000132588

Source DB:  PubMed          Journal:  Cytogenet Cell Genet        ISSN： 0301-0171


  9 in total

1.  Molecular genetic analysis of DNA obtained from fixed, air dried or paraffin embedded sources.

Authors:  K Grünewald; J Lyons; T E Hansen-Hagge; J W Janssen; H Feichtinger; C R Bartram
Journal:  Ann Hematol       Date:  1991-04       Impact factor: 3.673

2.  Band-specific localization of the microsatellite at D13S71 by microdissection and enzymatic amplification.

Authors:  H Spielvogel; H C Hennies; U Claussen; S S Washington; A Chakravarti; A Reis
Journal:  Am J Hum Genet       Date:  1992-05       Impact factor: 11.025

Review 3.  Restriction endonucleases in the study of eukaryotic chromosomes.

Authors:  C López-Fernández; J Gosálvez; L Ferrucci; R Mezzannotte
Journal:  Genetica       Date:  1991       Impact factor: 1.082

4.  Patterns of digestion of human chromosomes by restriction endonucleases demonstrated by in situ nick translation.

Authors:  A T Sumner; M H Taggart; R Mezzanotte; L Ferrucci
Journal:  Histochem J       Date:  1990-12

5.  Microdissection of banded human chromosomes.

Authors:  G Senger; H J Lüdecke; B Horsthemke; U Claussen
Journal:  Hum Genet       Date:  1990-05       Impact factor: 4.132

6.  A simple and efficient method for microdissection and microFISH.

Authors:  J J Engelen; J C Albrechts; G J Hamers; J P Geraedts
Journal:  J Med Genet       Date:  1998-04       Impact factor: 6.318

7.  Longitudinal patterns similar to G-banding in untreated human chromosomes: evidence from atomic force microscopy.

Authors:  A Musio; T Mariani; C Frediani; I Sbrana; C Ascoli
Journal:  Chromosoma       Date:  1994-06       Impact factor: 4.316

8.  Delineation of marker chromosomes by reverse chromosome painting using only a small number of DOP-PCR amplified microdissected chromosomes.

Authors:  R Viersbach; G Schwanitz; M M Nöthen
Journal:  Hum Genet       Date:  1994-06       Impact factor: 4.132

9.  The origin of cytologically unidentifiable chromosome abnormalities: six cases ascertained by targeted chromosome-band painting.

Authors:  T Ohta; T Tohma; H Soejima; Y Fukushima; T Nagai; K Yoshiura; Y Jinno; N Niikawa
Journal:  Hum Genet       Date:  1993-08       Impact factor: 4.132
  9 in total

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