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Literature DB >> 2845398

Molecular cloning and expression of the cDNA for the hamster alpha 1-adrenergic receptor.

S Cotecchia¹, D A Schwinn, R R Randall, R J Lefkowitz, M G Caron, B K Kobilka.

Abstract

The cDNA for the Syrian hamster alpha 1-adrenergic receptor has been cloned with oligonucleotides corresponding to the partial amino acid sequence of the receptor protein purified from DDT1MF-2 smooth muscle cells. The deduced amino acid sequence encodes a 515-residue polypeptide that shows the most sequence identity with the other adrenergic receptors and the putative protein product of the related clone G-21. Similarities with the muscarinic cholinergic receptors are also evident. Expression studies in COS-7 cells confirm that we have cloned the alpha 1-adrenergic receptor that couples to inositol phospholipid metabolism.

Entities: Chemical Gene Species

Mesh：

Substances：

Year: 1988 PMID： 2845398 PMCID： PMC282143 DOI： 10.1073/pnas.85.19.7159

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

33 in total

1. Induction of DNA synthesis in cultured rat hepatocytes through stimulation of alpha 1 adrenoreceptor by norepinephrine.

Authors: J L Cruise; K A Houck; G K Michalopoulos
Journal: Science Date: 1985-02-15 Impact factor: 47.728

Review 2. Inositol trisphosphate and diacylglycerol as second messengers.

Authors: M J Berridge
Journal: Biochem J Date: 1984-06-01 Impact factor: 3.857

Review 3. Molecular mechanisms involved in alpha-adrenergic responses.

Authors: J H Exton
Journal: Mol Cell Endocrinol Date: 1981-09 Impact factor: 4.102

4. Photoaffinity labeling of mammalian alpha 1-adrenergic receptors. Identification of the ligand binding subunit with a high affinity radioiodinated probe.

Authors: L M Leeb-Lundberg; K E Dickinson; S L Heald; J E Wikberg; P O Hagen; J F DeBernardis; M Winn; D L Arendsen; R J Lefkowitz; M G Caron
Journal: J Biol Chem Date: 1984-02-25 Impact factor: 5.157

5. A simple method for displaying the hydropathic character of a protein.

Authors: J Kyte; R F Doolittle
Journal: J Mol Biol Date: 1982-05-05 Impact factor: 5.469

6. Validation and statistical analysis of a computer modeling method for quantitative analysis of radioligand binding data for mixtures of pharmacological receptor subtypes.

Authors: A De Lean; A A Hancock; R J Lefkowitz
Journal: Mol Pharmacol Date: 1982-01 Impact factor: 4.436

Molecular cloning and expression of the cDNA for the hamster alpha 1-adrenergic receptor.

1. Induction of DNA synthesis in cultured rat hepatocytes through stimulation of alpha 1 adrenoreceptor by norepinephrine.

Review 2. Inositol trisphosphate and diacylglycerol as second messengers.

Review 3. Molecular mechanisms involved in alpha-adrenergic responses.

4. Photoaffinity labeling of mammalian alpha 1-adrenergic receptors. Identification of the ligand binding subunit with a high affinity radioiodinated probe.

5. A simple method for displaying the hydropathic character of a protein.

6. Validation and statistical analysis of a computer modeling method for quantitative analysis of radioligand binding data for mixtures of pharmacological receptor subtypes.

7. Cloning and expression of a human kidney cDNA for an alpha 2-adrenergic receptor subtype.

8. Characterization of the alpha 1-adrenergic receptor subtype in a smooth muscle cell line.

9. Catecholamines are mitogenic in 3T3 and bovine aortic endothelial cells.

10. The labelling of proteins to high specific radioactivities by conjugation to a 125I-containing acylating agent.

1. Investigation of the subtypes of alpha1-adrenoceptor mediating contractions of rat vas deferens.

2. Distribution of alpha-1 adrenoceptor subtypes in RNA and protein in rabbit eyes.

3. Effects of castration on contraction and alpha(1)-adrenoceptor expression in rat prostate.

Review 4. In vitro mutagenesis and the search for structure-function relationships among G protein-coupled receptors.

5. α(1A)-adrenergic receptor differentially regulates STAT3 phosphorylation through PKCϵ and PKCδ in myocytes.

6. RTA, a candidate G protein-coupled receptor: cloning, sequencing, and tissue distribution.

7. Network-based predictions of in vivo cardiac hypertrophy.

8. alpha1B-Adrenergic receptor phosphorylation and desensitization induced by transforming growth factor-beta.

9. Tunicamycin inhibits prostaglandin F2 alpha receptor-mediated phosphoinositide hydrolysis in cultured rat astrocytes.

Review 10. Classification of alpha 1-adrenoceptor subtypes.