R Sassi1, H Sahli2, E Cheour2, S Sellami2, A Ben Ammar El Gaaied1. 1. a Genetics, Immunology and Human Pathologies Laboratory, Faculty of Mathematical, Physical and Natural Sciences of Tunis , Tunis EL Manar University , Tunis , Tunisia. 2. b Immuno-Rheumatology Laboratory , Rabta Hospital, Faculty of Medicine of Tunis, Tunis EL Manar University , Tunis , Tunisia.
Abstract
OBJECTIVES: The dynamic nature of the skeleton is achieved by a remodeling process. Receptor activator of nuclear factor kappa B (RANK) ligand (RANKL) stimulates bone resorption by activating RANK signaling. Therefore it is considered as a candidate gene regulating susceptibility to osteoporosis. In the current study, we have investigated the association between the RANKL gene -693G > C and -643 C > T polymorphisms and bone mineral density (BMD) in a population of postmenopausal Tunisian women. METHODS: Polymorphic sites in RANKL gene (rs9533155 -693G > C and rs9533156 -643 C > T polymorphisms) were determined using PCR-RFLP analysis in 566 postmenopausal Tunisian women. All statistical analysis were examined by SPSS software. RESULTS: We have detected a significant difference in lumbar spine and hip BMD for -643C > T genotypes. For -693G > C genotypes, a significant difference was detected only in hip BMD. The distribution of -643C > T genotypes and alleles between three groups (osteoporotic, osteopenic and normal women) revealed a significant association of the TT genotype with development of osteoporosis (p = 0.01; odds ratio 2.15), although for the -693G > C polymorphism, no significant results were found. CONCLUSION: We have demonstrated the association of the -643C > T polymorphism with BMD variation and osteoporosis risk in postmenopausal Tunisian women.
OBJECTIVES: The dynamic nature of the skeleton is achieved by a remodeling process. Receptor activator of nuclear factor kappa B (RANK) ligand (RANKL) stimulates bone resorption by activating RANK signaling. Therefore it is considered as a candidate gene regulating susceptibility to osteoporosis. In the current study, we have investigated the association between the RANKL gene -693G > C and -643 C > T polymorphisms and bone mineral density (BMD) in a population of postmenopausal Tunisian women. METHODS: Polymorphic sites in RANKL gene (rs9533155 -693G > C and rs9533156 -643 C > T polymorphisms) were determined using PCR-RFLP analysis in 566 postmenopausal Tunisian women. All statistical analysis were examined by SPSS software. RESULTS: We have detected a significant difference in lumbar spine and hip BMD for -643C > T genotypes. For -693G > C genotypes, a significant difference was detected only in hip BMD. The distribution of -643C > T genotypes and alleles between three groups (osteoporotic, osteopenic and normal women) revealed a significant association of the TT genotype with development of osteoporosis (p = 0.01; odds ratio 2.15), although for the -693G > C polymorphism, no significant results were found. CONCLUSION: We have demonstrated the association of the -643C > T polymorphism with BMD variation and osteoporosis risk in postmenopausal Tunisian women.
Authors: M Barake; R El Eid; S Ajjour; M Chakhtoura; L Meho; T Mahmoud; J Atieh; A M Sibai; G El-Hajj Fuleihan Journal: Osteoporos Int Date: 2021-04-07 Impact factor: 4.507
Authors: Beata Mrozikiewicz-Rakowska; Piotr Nehring; Konrad Szymański; Agnieszka Sobczyk-Kopcioł; Rafał Płoski; Wojciech Drygas; Janusz Krzymień; Nikita Amit Acharya; Leszek Czupryniak; Adam Przybyłkowski Journal: J Diabetes Metab Disord Date: 2018-11-09