Amel Abderrahim1,2, Nassima Djahmi3, Charlotte Pujol4, Sabina Nedjai3, Mohamed Cherif Bentakouk5, Djamila Kirane-Gacemi2, Mazouz Dekhil3, Albert Sotto1,6, Jean-Philippe Lavigne1,4, Alix Pantel1,4. 1. 1 Institut National de la Santé et de la Recherche Médicale, U1047, Université de Montpellier, UFR de Médecine , Nîmes, France . 2. 2 Laboratoire d'Amélioration Génétique des Plantes, Equipe Microorganismes et Environnement, Université Badji Mokhtar , Annaba, Algérie. 3. 3 Laboratoire de Microbiologie, CHU Ibn Rochd , Annaba, Algérie. 4. 4 Service de Microbiologie, CHU Carémeau , Nîmes, France . 5. 5 Centre de Réanimation et de Traitement des Brûlés, Hôpital Ibn-Sina, CHU Annaba , Annaba, Algérie. 6. 6 Service des Maladies Infectieuses et Tropicales, CHU Carémeau , Nîmes, France .
Abstract
AIM: The aim of this study was to characterize two carbapenem-resistant Klebsiella pneumoniae isolates recovered from urine samples in a patient hospitalized at Annaba University hospital (Algeria) in 2014. RESULTS: Two K. pneumoniae isolates were studied because they proved resistant to almost all antibiotics tested with a high level resistance to ertapenem (minimum inhibitory concentration = 32 mg/L). The results of modified Hodge test and combined disk test (ROSCO Diagnostica, Taastrup, Denmark) were positive. The two isolates harbored the blaNDM-1 gene and one was also positive for blaCTX-M-15. Screening of aminoglycoside-modifying enzymes and plasmid-mediated quinolone resistance contents detected aac(6')-Ib-cr, aac(3')-II, qnrB2, and oqxAB in both isolates. Multilocus sequence typing demonstrated that the two isolates belonged to sequence type 147. However, repetitive sequence-based PCR and pulsed-field gel electrophoresis showed that they were not clonally related. The blaNDM-1 gene and all other resistant genes were contained on an IncR plasmid of c.a. 85 kb. CONCLUSIONS: This study comprises the first identification of NDM-1-producing K. pneumoniae in Algeria. We thus confirm the concerning worldwide dissemination of this carbapenemase that involves the emergence of the IncR plasmid and the success of the ST147 clonal complex harboring it.
AIM: The aim of this study was to characterize two carbapenem-resistant Klebsiella pneumoniae isolates recovered from urine samples in a patient hospitalized at Annaba University hospital (Algeria) in 2014. RESULTS: Two K. pneumoniae isolates were studied because they proved resistant to almost all antibiotics tested with a high level resistance to ertapenem (minimum inhibitory concentration = 32 mg/L). The results of modified Hodge test and combined disk test (ROSCO Diagnostica, Taastrup, Denmark) were positive. The two isolates harbored the blaNDM-1 gene and one was also positive for blaCTX-M-15. Screening of aminoglycoside-modifying enzymes and plasmid-mediated quinolone resistance contents detected aac(6')-Ib-cr, aac(3')-II, qnrB2, and oqxAB in both isolates. Multilocus sequence typing demonstrated that the two isolates belonged to sequence type 147. However, repetitive sequence-based PCR and pulsed-field gel electrophoresis showed that they were not clonally related. The blaNDM-1 gene and all other resistant genes were contained on an IncR plasmid of c.a. 85 kb. CONCLUSIONS: This study comprises the first identification of NDM-1-producing K. pneumoniae in Algeria. We thus confirm the concerning worldwide dissemination of this carbapenemase that involves the emergence of the IncR plasmid and the success of the ST147 clonal complex harboring it.
Authors: Orsolya Szabo; Bela Kocsis; Nikolett Szabo; Katalin Kristof; Dora Szabo Journal: Can J Infect Dis Med Microbiol Date: 2018-09-23 Impact factor: 2.471
Authors: Yogandree Ramsamy; Koleka P Mlisana; Mushal Allam; Daniel G Amoako; Akebe L K Abia; Arshad Ismail; Ravesh Singh; Theroshnie Kisten; Khine Swe Han; David J Jackson Muckart; Timothy Hardcastle; Moosa Suleman; Sabiha Y Essack Journal: Microorganisms Date: 2020-01-17