| Literature DB >> 28428369 |
Alexandra-Chloé Villani1,2, Rahul Satija3,4,5, Gary Reynolds6, Siranush Sarkizova3, Karthik Shekhar3, James Fletcher6, Morgane Griesbeck7, Andrew Butler4,5, Shiwei Zheng4,5, Suzan Lazo8, Laura Jardine6, David Dixon6, Emily Stephenson6, Emil Nilsson9, Ida Grundberg9, David McDonald6, Andrew Filby6, Weibo Li3,2, Philip L De Jager3,10, Orit Rozenblatt-Rosen3, Andrew A Lane3,8, Muzlifah Haniffa11,12, Aviv Regev1,13,14, Nir Hacohen1,2.
Abstract
Dendritic cells (DCs) and monocytes play a central role in pathogen sensing, phagocytosis, and antigen presentation and consist of multiple specialized subtypes. However, their identities and interrelationships are not fully understood. Using unbiased single-cell RNA sequencing (RNA-seq) of ~2400 cells, we identified six human DCs and four monocyte subtypes in human blood. Our study reveals a new DC subset that shares properties with plasmacytoid DCs (pDCs) but potently activates T cells, thus redefining pDCs; a new subdivision within the CD1C+ subset of DCs; the relationship between blastic plasmacytoid DC neoplasia cells and healthy DCs; and circulating progenitor of conventional DCs (cDCs). Our revised taxonomy will enable more accurate functional and developmental analyses as well as immune monitoring in health and disease.Entities:
Mesh：
Year: 2017 PMID： 28428369 PMCID： PMC5775029 DOI： 10.1126/science.aah4573
Source DB: PubMed Journal: Science ISSN： 0036-8075 Impact factor: 47.728