Draft Genome Sequences of Three Listeria monocytogenes Isolates from Foods in China.

Listeria monocytogenes is a foodborne pathogen of global concern because of the high mortality rate among patients. The draft genome sequences of three L. monocytogenes strains isolated from foods are reported here. The availability of these genomes should provide useful information on the genomic diversity of L. monocytogenes isolated from foods in China.

GENOME ANNOUNCEMENT

Listeria monocytogenes is an important foodborne pathogen causing severe illness, such as septicemia and meningitis, among pregnant women, elderly people, and immunocompromised individuals (1, 2). L. monocytogenes is ubiquitous and has been reportedly isolated from a wide range of foods, including meat, poultry, dairy products, fruit, and vegetables (3, 4). Whole-genome sequencing has become an essential tool for investigating the evolution, diversity, and pathogenicity of foodborne bacterial pathogens (5–9). The draft genome sequences of three L. monocytogenes strains from foods in the Shanghai area of China are reported here.

Genomic DNA was isolated from overnight cultures and sequenced using MiSeq (Illumina, San Diego, CA) to obtain draft genomes. Genomic data were assembled using SOAPdenovo version 2 (10), including the serotype 1/2c strain SHL12-2 (16 contigs, 3.01 Mb, 583,457 bp N50 contig size, and 2,978 identified genes), serotype 1/2a strain SHL12-22 (20 contigs, 3.02 Mb, 506,776 bp N50 contig size, and 2,943 identified genes), and serotype 1/2a strain SHL13-12 (17 contigs, 2.99 Mb, 524,215 bp N50 contig size, and 2,884 identified genes). These sequences were annotated using the NCBI Prokaryotic Genome Annotation Pipeline (11).

Accession number(s).

These three draft genome sequences have been deposited in GenBank under the accession numbers LRTW00000000, LRTX00000000, and LRTY00000000.