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Literature DB >> 2841647

Analysis of the psoralen-crosslinking pattern in chromatin DNA by exonuclease digestion.

Abstract

When chromatin is photoreacted with psoralen, crosslinks occur preferentially in the linker DNA between nucleosomes. The pattern of these crosslinks can be analysed by exonuclease digestion of random DNA fragments, since the exonucleases tested stop at sites of psoralen-crosslinks. Further digestion of these fragments with S1-nuclease leads to DNA fragments of nucleosomal and polynucleosomal size, which presumably carry psoralen-crosslinks at both ends. This method of analysis of chromatin structure complements the classical micrococcal nuclease digestion analysis, since it can be performed in vitro as well as in vivo, and since it is independent of pH and ionic conditions.

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Year: 1988 PMID： 2841647 PMCID： PMC338348 DOI： 10.1093/nar/16.14.7013

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

16 in total

1. Micrococcal nuclease as a DNA structural probe: its recognition sequences, their genomic distribution and correlation with DNA structure determinants.

Authors: J T Flick; J C Eissenberg; S C Elgin
Journal: J Mol Biol Date: 1986-08-20 Impact factor: 5.469

2. Location of the primary sites of micrococcal nuclease cleavage on the nucleosome core.

Authors: M Cockell; D Rhodes; A Klug
Journal: J Mol Biol Date: 1983-10-25 Impact factor: 5.469

3. Interstrand psoralen cross-links do not introduce appreciable bends in DNA.

Authors: R R Sinden; P J Hagerman
Journal: Biochemistry Date: 1984-12-18 Impact factor: 3.162

4. Photochemistry of the furan-side 8-methoxypsoralen-thymidine monoadduct inside the DNA helix. Conversion to diadduct and to pyrone-side monoadduct.

Authors: J W Tessman; S T Isaacs; J E Hearst
Journal: Biochemistry Date: 1985-03-26 Impact factor: 3.162

5. Structural changes of soluble rat liver chromatin induced by the shift in pH from 7 to 9.

Authors: P Labhart; F Thoma; T Koller
Journal: Eur J Cell Biol Date: 1981-08 Impact factor: 4.492

6. Sharp kink of DNA at psoralen-cross-link site deduced from crystal structure of psoralen-thymine monoadduct.

Authors: S H Kim; S Peckler; B Graves; D Kanne; H Rapoport; J E Hearst
Journal: Cold Spring Harb Symp Quant Biol Date: 1983

7. Structure of in-vivo transcribing chromatin as studied in simian virus 40 minichromosomes.

Authors: W De Bernardin; T Koller; J M Sogo
Journal: J Mol Biol Date: 1986-10-05 Impact factor: 5.469

8. Structure of replicating simian virus 40 minichromosomes. The replication fork, core histone segregation and terminal structures.

Authors: J M Sogo; H Stahl; T Koller; R Knippers
Journal: J Mol Biol Date: 1986-05-05 Impact factor: 5.469

9. Psoralen-crosslinking of DNA as a probe for the structure of active nucleolar chromatin.

Authors: J M Sogo; P J Ness; R M Widmer; R W Parish; T Koller
Journal: J Mol Biol Date: 1984-10-05 Impact factor: 5.469

10. Psoralen-crosslinking of soluble and of H1-depleted soluble rat liver chromatin.

Authors: A Conconi; R Losa; T Koller; J M Sogo
Journal: J Mol Biol Date: 1984-10-05 Impact factor: 5.469

8 in total

1. Ribosomal gene clusters are uniquely proportioned between open and closed chromatin structures in both tomato leaf cells and exponentially growing suspension cultures.

Authors: A Conconi; J M Sogo; C A Ryan
Journal: Proc Natl Acad Sci U S A Date: 1992-06-15 Impact factor: 11.205

Analysis of the psoralen-crosslinking pattern in chromatin DNA by exonuclease digestion.

1. Micrococcal nuclease as a DNA structural probe: its recognition sequences, their genomic distribution and correlation with DNA structure determinants.

2. Location of the primary sites of micrococcal nuclease cleavage on the nucleosome core.

3. Interstrand psoralen cross-links do not introduce appreciable bends in DNA.

4. Photochemistry of the furan-side 8-methoxypsoralen-thymidine monoadduct inside the DNA helix. Conversion to diadduct and to pyrone-side monoadduct.

5. Structural changes of soluble rat liver chromatin induced by the shift in pH from 7 to 9.

6. Sharp kink of DNA at psoralen-cross-link site deduced from crystal structure of psoralen-thymine monoadduct.

7. Structure of in-vivo transcribing chromatin as studied in simian virus 40 minichromosomes.

8. Structure of replicating simian virus 40 minichromosomes. The replication fork, core histone segregation and terminal structures.

9. Psoralen-crosslinking of DNA as a probe for the structure of active nucleolar chromatin.

10. Psoralen-crosslinking of soluble and of H1-depleted soluble rat liver chromatin.

1. Ribosomal gene clusters are uniquely proportioned between open and closed chromatin structures in both tomato leaf cells and exponentially growing suspension cultures.

2. Histone acetylation facilitates RNA polymerase II transcription of the Drosophila hsp26 gene in chromatin.

3. Light affects the structure of Chlamydomonas chloroplast chromosomes.

4. Nucleosome positioning at the replication fork.

5. Intercalation of psoralen into DNA of plastid chromosomes decreases late during barley chloroplast development.

6. Different chromatin structures along the spacers flanking active and inactive Xenopus rRNA genes.

7. Histone-DNA interactions in the chromatin of procyclic Trypanosoma brucei brucei.

8. Chromatin structures and transcription of rDNA in yeast Saccharomyces cerevisiae.