| Literature DB >> 28409117 |
A A Pochtovyy1,2, O Yu Baranov3, I E Rubel'3, O A Razumova3, V E Padutov3, A V Khromov4, A V Makhotenko4, A P Tkachuk1, V V Makarov5, V A Gushchin1,5.
Abstract
The most common type of coniferous mobile genetic elements are retrotransposons. Despite of their early positive impact on evolution of modern coniferous species they can have a significant negative impact for Forestry and breeding. Breaking genomic structural integrity mobile elements can cause phenotypic defects of plants. In this regard, the study of the diversity of coniferous mobile genetic elements is particularly interesting. In the present paper, we describe mobile genetic elements in dsRNA fraction of Pinus sylvestris having witches broom abnormalities. In result of assembled contigs analysis by RepeatMasker 70 mobile genetic elements were identified. A 68 of that were retroelements. Most of elements represented by Gypsy (16 contigs) and Copia (48 contigs). In 4 cases retroelements specific to Pinus taeda were identified. In most cases fragments of integrase (24), reverse transcriptase (22) and RNaseH (15) were identified. Results of the study may be of interest for coniferous breeding and genetic specialists. The raw data of these experiments have been deposited at NCBI under the accession number SAMN06185845.Entities:
Keywords: Mobile genetic elements; NGS; dsRNA
Year: 2017 PMID： 28409117 PMCID： PMC5382025 DOI： 10.1016/j.gdata.2017.03.010
Source DB: PubMed Journal: Genom Data ISSN： 2213-5960