| Literature DB >> 28395805 |
Dongrui Ma1, Shin Hui Ng2, Li Zeng2, Yi Zhao3, Eng King Tan4.
Abstract
Peripheral blood mononuclear cells (PBMCs) were collected from a clinically diagnosed 64-year old male Parkinson's disease (PD) patient with S1647T variant in the LRRK2 gene. The PMBCs were reprogrammed with the human OSKM transcription factors using the Sendai-virus reprogramming system. The transgene-free iPSC showed pluripotency confirmed by immunofluorescent staining for pluripotency markers and differentiated into the 3 germ layers in vivo. The iPSC line also showed normal karyotype. This cellular model will be useful for further function studies and therapeutic screening.Entities:
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Year: 2016 PMID： 28395805 DOI： 10.1016/j.scr.2016.12.010
Source DB: PubMed Journal: Stem Cell Res ISSN： 1873-5061 Impact factor: 2.020