| Literature DB >> 28388409 |
Yang Yang1, Bei Liu1, Jun Xu2, Jinlin Wang2, Jun Wu3, Cheng Shi4, Yaxing Xu5, Jiebin Dong2, Chengyan Wang2, Weifeng Lai5, Jialiang Zhu2, Liang Xiong5, Dicong Zhu1, Xiang Li2, Weifeng Yang6, Takayoshi Yamauchi3, Atsushi Sugawara3, Zhongwei Li3, Fangyuan Sun7, Xiangyun Li7, Chen Li8, Aibin He8, Yaqin Du2, Ting Wang2, Chaoran Zhao2, Haibo Li2, Xiaochun Chi9, Hongquan Zhang9, Yifang Liu10, Cheng Li11, Shuguang Duo12, Ming Yin6, Huan Shen13, Juan Carlos Izpisua Belmonte14, Hongkui Deng15.
Abstract
Of all known cultured stem cell types, pluripotent stem cells (PSCs) sit atop the landscape of developmental potency and are characterized by their ability to generate all cell types of an adult organism. However, PSCs show limited contribution to the extraembryonic placental tissues in vivo. Here, we show that a chemical cocktail enables the derivation of stem cells with unique functional and molecular features from mice and humans, designated as extended pluripotent stem (EPS) cells, which are capable of chimerizing both embryonic and extraembryonic tissues. Notably, a single mouse EPS cell shows widespread chimeric contribution to both embryonic and extraembryonic lineages in vivo and permits generating single-EPS-cell-derived mice by tetraploid complementation. Furthermore, human EPS cells exhibit interspecies chimeric competency in mouse conceptuses. Our findings constitute a first step toward capturing pluripotent stem cells with extraembryonic developmental potentials in culture and open new avenues for basic and translational research. VIDEO ABSTRACT.Entities:
Keywords: chimeric ability; embryonic and extraembryonic developmental potentials; interspecies chimeric competency; single-cell derived chimeras
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Year: 2017 PMID： 28388409 PMCID： PMC5679268 DOI： 10.1016/j.cell.2017.02.005
Source DB: PubMed Journal: Cell ISSN： 0092-8674 Impact factor: 41.582