Amanda Z Zucoloto1, Marília F Manchope1, Larrisa Staurengo-Ferrari1, José C Alves-Filho2, Thiago M Cunha2, Maísa M Antunes3, Gustavo B Menezes3, Fernando Q Cunha2, Rubia Casagrande4, Waldiceu A Verri5. 1. Departamento de Ciências Patológicas, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Caixa Postal 10.011, Rod. Celso Garcia Cid PR445 km 480, Londrina, Paraná, CEP 86057-970, Brazil. 2. Department of Pharmacology, Ribeirão Preto Medical School, University of São Paulo, Av. Bandeirantes 3900, Ribeirão Preto, São Paulo, CEP 14049-900, Brazil. 3. Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Avenida Presidente Antonio Carlos 6627, Belo Horizonte, Minas Gerais, CEP 31270-901, Brazil. 4. Departamento de Ciências Farmacêuticas, Centro de Ciências da Saúde, Universidade Estadual de Londrina, Av. Robert Koch, 60, Londrina, Paraná, CEP 86038-350, Brazil. 5. Departamento de Ciências Patológicas, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Caixa Postal 10.011, Rod. Celso Garcia Cid PR445 km 480, Londrina, Paraná, CEP 86057-970, Brazil. waldiceujr@yahoo.com.br.
Abstract
OBJECTIVE AND DESIGN: This study aimed to evaluate the effect of probucol in inflammatory hyperalgesia and leukocyte recruitment in mice. TREATMENT: Probucol at 0.3-3 mg/kg was administrated per oral 1 h before inflammatory stimulus.Author: Kindly check and confirm the affiliation 1 have been correctly processed or not and amend if necessary.Thank you. We have corrected affiliation 1. We added the information to the appropriate boxes. However the state and the postal code are in a different order when compared to the other affiliations. METHODS: Overt pain-like behaviors were determined by the number of abdominal writhings induced by phenyl-p-benzoquinone and acetic acid. Mechanical and thermal hyperalgesia induced by carrageenan were determined using an electronic anesthesiometer and hot plate apparatus, respectively. Leukocyte recruitment was evaluated by direct count or by determination of myeloperoxidase and N-acetylglucosaminidase activities. Antioxidant ability was determined by measurement of GSH levels, ABTS and FRAP assays. Cytokine production and NF-кB activation were evaluated by ELISA. Data were analyzed by ANOVA followed by Tukey's post-hoc. p < 0.05 was considered significant. RESULTS: Probucol reduced overt pain-like behavior, and carrageenan-induced mechanical and thermal hyperalgesia. These effects were accompanied by reduced leukocyte influx in both paw skin and peritoneum exudate. Probucol did not alter carrageenan-induced tissue antioxidant capacity at anti-inflammatory/analgesic dose. On the other hand, probucol inhibited carrageenan-induced IL-1β, TNF-α and CXCL1 production as well as NF-кB activation. CONCLUSION: Probucol presents analgesic and anti-inflammatory activities by employing mechanisms other than its antioxidant properties. These mechanisms involve targeting of pro-inflammatory cytokines and NF-кB activation.
OBJECTIVE AND DESIGN: This study aimed to evaluate the effect of probucol in inflammatory hyperalgesia and leukocyte recruitment in mice. TREATMENT: Probucol at 0.3-3 mg/kg was administrated per oral 1 h before inflammatory stimulus.Author: Kindly check and confirm the affiliation 1 have been correctly processed or not and amend if necessary.Thank you. We have corrected affiliation 1. We added the information to the appropriate boxes. However the state and the postal code are in a different order when compared to the other affiliations. METHODS: Overt pain-like behaviors were determined by the number of abdominal writhings induced by phenyl-p-benzoquinone and acetic acid. Mechanical and thermal hyperalgesia induced by carrageenan were determined using an electronic anesthesiometer and hot plate apparatus, respectively. Leukocyte recruitment was evaluated by direct count or by determination of myeloperoxidase and N-acetylglucosaminidase activities. Antioxidant ability was determined by measurement of GSH levels, ABTS and FRAP assays. Cytokine production and NF-кB activation were evaluated by ELISA. Data were analyzed by ANOVA followed by Tukey's post-hoc. p < 0.05 was considered significant. RESULTS:Probucol reduced overt pain-like behavior, and carrageenan-induced mechanical and thermal hyperalgesia. These effects were accompanied by reduced leukocyte influx in both paw skin and peritoneum exudate. Probucol did not alter carrageenan-induced tissue antioxidant capacity at anti-inflammatory/analgesic dose. On the other hand, probucol inhibited carrageenan-induced IL-1β, TNF-α and CXCL1 production as well as NF-кB activation. CONCLUSION:Probucol presents analgesic and anti-inflammatory activities by employing mechanisms other than its antioxidant properties. These mechanisms involve targeting of pro-inflammatory cytokines and NF-кB activation.
Authors: L S Barcelos; A Talvani; A S Teixeira; L Q Vieira; G D Cassali; S P Andrade; M M Teixeira Journal: J Leukoc Biol Date: 2005-05-13 Impact factor: 4.962
Authors: Alexander M Binshtok; Haibin Wang; Katharina Zimmermann; Fumimasa Amaya; Daniel Vardeh; Lin Shi; Gary J Brenner; Ru-Rong Ji; Bruce P Bean; Clifford J Woolf; Tarek A Samad Journal: J Neurosci Date: 2008-12-24 Impact factor: 6.167
Authors: Saud Bawazeer; Dalia Farag A El-Telbany; Majid Mohammad Al-Sawahli; Gamal Zayed; Ahmed Abdallah A Keed; Abdelaziz E Abdelaziz; Doaa H Abdel-Naby Journal: Drug Deliv Date: 2020-12 Impact factor: 6.419