| Literature DB >> 28385729 |
Adam Jozwiak1,2,3,4, Agata Lipko1,2,3,4, Magdalena Kania1,2,3,4, Witold Danikiewicz1,2,3,4, Liliana Surmacz1,2,3,4, Agnieszka Witek1,2,3,4, Jacek Wojcik1,2,3,4, Konrad Zdanowski1,2,3,4, Cezary Pączkowski1,2,3,4, Tadeusz Chojnacki1,2,3,4, Jaroslaw Poznanski5,6,7,8, Ewa Swiezewska5,6,7,8.
Abstract
The cooperation of the mevalonate (MVA) and methylerythritol phosphate (MEP) pathways, operating in parallel in plants to generate isoprenoid precursors, has been studied extensively. Elucidation of the isoprenoid metabolic pathways is indispensable for the rational design of plant and microbial systems for the production of industrially valuable terpenoids. Here, we describe a new method, based on numerical modeling of mass spectra of metabolically labeled dolichols (Dols), designed to quantitatively follow the cooperation of MVA and MEP reprogrammed upon osmotic stress (sorbitol treatment) in Arabidopsis (Arabidopsis thaliana). The contribution of the MEP pathway increased significantly (reaching 100%) exclusively for the dominating Dols, while for long-chain Dols, the relative input of the MEP and MVA pathways remained unchanged, suggesting divergent sites of synthesis for dominating and long-chain Dols. The analysis of numerically modeled Dol mass spectra is a novel method to follow modulation of the concomitant activity of isoprenoid-generating pathways in plant cells; additionally, it suggests an exchange of isoprenoid intermediates between plastids and peroxisomes.Entities:
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Year: 2017 PMID： 28385729 PMCID： PMC5462023 DOI： 10.1104/pp.17.00036
Source DB: PubMed Journal: Plant Physiol ISSN： 0032-0889 Impact factor: 8.340