Arij Mechergui1,2, Wafa Achour1,2, Assia Ben Hassen1,2. 1. The National Bone Marrow Transplant Centre, Tunis, Tunisia. 2. Faculty of Medicine of Tunis, UR12ES02, Tunis, Tunisia.
Abstract
BACKGROUND: We investigated the diversity of the primary sequences of the 16S rRNA genes among 46 commensal Neisseria strains and evaluated the use of this approach as a molecular typing tool in comparison with PFGE analysis. METHODS: Identification to the genus was done using conventional methods and API NH (bio-Mérieux® ). Identification to species level was based on 16S rRNA gene sequencing. PFGE analysis was done using SpeI. RESULTS: Fourteen, two, three and fourteen 16S rRNA sequence types were found among twenty Neisseria flavescens, two Neisseria sicca, five Neisseria macacae and nineteen Neisseria mucosa clinical isolates. Forty-three different PFGE patterns were found among the tested strains. CONCLUSION: We demonstrated a high diversity among 16S rRNA genes which was reflected by PFGE analysis.
BACKGROUND: We investigated the diversity of the primary sequences of the 16S rRNA genes among 46 commensal Neisseria strains and evaluated the use of this approach as a molecular typing tool in comparison with PFGE analysis. METHODS: Identification to the genus was done using conventional methods and API NH (bio-Mérieux® ). Identification to species level was based on 16S rRNA gene sequencing. PFGE analysis was done using SpeI. RESULTS: Fourteen, two, three and fourteen 16S rRNA sequence types were found among twenty Neisseria flavescens, two Neisseria sicca, five Neisseria macacae and nineteen Neisseria mucosa clinical isolates. Forty-three different PFGE patterns were found among the tested strains. CONCLUSION: We demonstrated a high diversity among 16S rRNA genes which was reflected by PFGE analysis.
Authors: Claudio T Sacchi; Anne M Whitney; Michael W Reeves; Leonard W Mayer; Tanja Popovic Journal: J Clin Microbiol Date: 2002-12 Impact factor: 5.948
Authors: T Popovic; C T Sacchi; M W Reeves; A M Whitney; L W Mayer; C A Noble; G W Ajello; F Mostashari; N Bendana; J Lingappa; R Hajjeh; N E Rosenstein Journal: Emerg Infect Dis Date: 2000 Jul-Aug Impact factor: 6.883