| Literature DB >> 28374152 |
César Andrés Romero1, Aline Remor2, Alexandra Latini2, Ana Lucía De Paul1, Alicia Inés Torres1, Jorge Humberto Mukdsi3.
Abstract
Uric acid (UA) has been associated with renal fibrosis and progression of chronic kidney disease. However, the underlying mechanisms of this process have still not been identified. Here, we studied the role of the innate imunity receptor NLRP3/ASC in UA induced epithelial-mesenchymal transition (EMT) in kidney. Wistar rats were fed with oxonic acid 2% and UA 2% (OXA + U), OXA + U plus allopurinol (ALL) or regular chow (C) for 7 weeks. We analyzed the presence of EMT markers, the expression of NLRP3, ASC, Caspase-1 and Smad 2/3 molecules and the mitochondrial morphological and functional characteristics. High UA induced renal fibrosis, mild chronic inflammation, as well as morphological and biochemical evidence of EMT. High UA also increased the expression of NLRP3/ASC with activation of both inflammasome related caspase-1 and inflammasome unrelated Smad 2/3 pathways. Ultrastructural co-localization of NLRP3 and Smad 2/3 indicated physical interaction between the two molecules. No morphological or functional changes were found between mitochondria exposed to high UA. In conclusion, kidney epithelial NLRP3/ASC expression was increased in high UA state in rats and both inflammasome related caspase-1 and non-inflammasome related P-Smad 2/3 pathways were associated with the observed EMT, inflammation and fibrosis induced by UA in the kidney.Entities:
Keywords: Epithelial-mesenchymal transition; Kidney; Mitochondria; NLRP3/ASC; Smad 2/3; Uric acid
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Year: 2017 PMID： 28374152 DOI： 10.1007/s10735-017-9720-9
Source DB: PubMed Journal: J Mol Histol ISSN： 1567-2379 Impact factor: 2.611