Background: The progressive decline in tissue mechanical strength that occurs with aging is hypothesized to be due to a loss of resident stem cell number and function. As such, there is concern regarding use of autologous adult stem cell therapy in older patients. To abrogate this, many patients elect to cryopreserve the adipose stromal-vascular fraction (SVF) of lipoaspirate, which contains resident adipose stem cells (ASC). However, it is not clear yet if there is any clinical benefit from banking cells at a younger age. Objectives: We performed a comparative analysis of SVF composition and ASC function from cells obtained under GMP conditions from the same three patients with time gap of 7 to 12 years. Methods: SVF, cryobanked under good manufacturing practice (GMP) conditions, was thawed and cell yield, viability, and cellular composition were assessed. In parallel, ASC proliferation and efficiency of tri-lineage differentiation were evaluated. Results: The results showed no significant differences existed in cell yield and SVF subpopulation composition within the same patient between harvest procedures 7 to 12 years apart. Further, no change in proliferation rates of cultured ASCs was found, and expanded cells from all patients were capable of tri-lineage differentiation. Conclusions: By harvesting fat from the same patient at two time points, we have shown that despite the natural human aging process, the prevalence and functional activity of ASCs in an adult mesenchymal stem cell, is highly preserved. Level of Evidence: 5.
Background: The progressive decline in tissue mechanical strength that occurs with aging is hypothesized to be due to a loss of resident stem cell number and function. As such, there is concern regarding use of autologous adult stem cell therapy in older patients. To abrogate this, many patients elect to cryopreserve the adipose stromal-vascular fraction (SVF) of lipoaspirate, which contains resident adipose stem cells (ASC). However, it is not clear yet if there is any clinical benefit from banking cells at a younger age. Objectives: We performed a comparative analysis of SVF composition and ASC function from cells obtained under GMP conditions from the same three patients with time gap of 7 to 12 years. Methods: SVF, cryobanked under good manufacturing practice (GMP) conditions, was thawed and cell yield, viability, and cellular composition were assessed. In parallel, ASC proliferation and efficiency of tri-lineage differentiation were evaluated. Results: The results showed no significant differences existed in cell yield and SVF subpopulation composition within the same patient between harvest procedures 7 to 12 years apart. Further, no change in proliferation rates of cultured ASCs was found, and expanded cells from all patients were capable of tri-lineage differentiation. Conclusions: By harvesting fat from the same patient at two time points, we have shown that despite the natural human aging process, the prevalence and functional activity of ASCs in an adult mesenchymal stem cell, is highly preserved. Level of Evidence: 5.
Authors: Darren G Haskett; Kamiel S Saleh; Katherine L Lorentz; Alexander D Josowitz; Samuel K Luketich; Justin S Weinbaum; Lauren E Kokai; Antonio D'Amore; Kacey G Marra; J Peter Rubin; William R Wagner; David A Vorp Journal: J Thorac Cardiovasc Surg Date: 2018-07-02 Impact factor: 5.209
Authors: Dina Rady; Marwa M S Abbass; Aiah A El-Rashidy; Sara El Moshy; Israa Ahmed Radwan; Christof E Dörfer; Karim M Fawzy El-Sayed Journal: Stem Cells Int Date: 2020-08-11 Impact factor: 5.443
Authors: Faina Linkov; Sharon L Goughnour; Shalkar Adambekov; Anna Lokshin; Joseph L Kelley; Paniti Sukumvanich; John T Comerci; Kacey G Marra; Lauren E Kokai; J Peter Rubin; Anda M Vlad; Brian J Philips; Robert P Edwards Journal: Biomark Med Date: 2018-07-25 Impact factor: 2.851
Authors: Tatiana Chadid; Andrew Morris; Alexandra Surowiec; Scott Robinson; Maiko Sasaki; Jacques Galipeau; Brian P Pollack; Luke P Brewster Journal: J Vasc Surg Date: 2018-08-10 Impact factor: 4.268
Authors: Önder Solakoglu; Werner Götz; Maren C Kiessling; Christopher Alt; Christoph Schmitz; Eckhard U Alt Journal: World J Stem Cells Date: 2019-02-26 Impact factor: 5.326