Leonardo Perez Faverani1, Tárik Ocon Braga Polo2, Gabriel Ramalho-Ferreira2, Gustavo Antonio Correa Momesso2, Jaqueline Suemi Hassumi2,3, Ana Cláudia Rossi4, Alexandre Rodrigues Freire4, Felippe Bevilacqua Prado4, Eloá Rodrigues Luvizuto5, Reinhard Gruber6, Roberta Okamoto3. 1. Department of Surgery and Integrated Clinic, Division of Oral and Maxillofacial Surgery, São Paulo State University (UNESP), School of Dentistry, Araçatuba, São Paulo, 16015-050, Brazil. leobucomaxilo@gmail.com. 2. Department of Surgery and Integrated Clinic, Division of Oral and Maxillofacial Surgery, São Paulo State University (UNESP), School of Dentistry, Araçatuba, São Paulo, 16015-050, Brazil. 3. Department of Basic Sciences, São Paulo State University (UNESP), School of Dentistry, Araçatuba, São Paulo, 16015-050, Brazil. 4. Department of Morphology, Piracicaba Dental School, University of Campinas (UNICAMP), Piracicaba, SP, Brazil. 5. Department of Surgery and Integrated Clinic, Division of Integrated Clinic, São Paulo State University UNESP), School of Dentistry, Araçatuba, São Paulo, 16015-050, Brazil. 6. Department of Oral Biology, Medical University of Vienna, Wien, Austria.
Abstract
OBJECTIVES: Alendronate and raloxifene, a bisphosphonate and a selective estrogen modulator, respectively, are established osteoporosis therapies. Current evidence suggests that simultaneous application of osteoporosis therapies modulates osseointegration. However, alendronate shows inconsistent findings and raloxifene has not been studied comprehensively. This study aimed to evaluate the bone dynamics and molecular and microstructural features at the peri-implant bone interface in osteoporotic rats. MATERIALS AND METHODS: Thirty female rats underwent ovariectomy and were fed a diet low in calcium and phosphate and treated with alendronate or raloxifene for 30 days or underwent fictional ovariectomy surgery (SHAM) prior to implant insertion in the tibia; osteoporosis therapies continued thereafter. After 42 days, peri-implant bone was evaluated by histometric and micro-CT analysis. Fluorochrome incorporation and gene expression was determined to evaluate bone turnover. RESULTS: We report here that alendronate had no impact on bone-to-implant contacts and the mineral apposition rate. The RANKL/OPG ratio and local bone volume, however, were increased compared to the untreated osteoporotic rats. Even though signaling to bone resorption activity through RANKL production was observed in the alendronate group, the blockade of bone resorption activity that occurs in decorrence to alendronate activity took place and resulted in an increase in bone volume. Raloxifene significantly increased osseointegration in osteoporotic rats, as indicated by bone-to-implant contacts, mineral apposition, and local bone volume. Raloxifene, however, had no considerable impact on the RANKL/OPG ratio compared to untreated osteoporotic rats. As expected, the SH group showed higher bone-to-implant contacts and mineral apposition rates than the untreated osteoporotic rats. CONCLUSIONS: These findings suggest that raloxifene but not alendronate can compensate for the impaired osseointegration in osteoporotic rats. CLINICAL RELEVANCE: Regarding the superiority of raloxifene observed in the improvement of bone dynamics response, this statement suggests that raloxifene could be a good option for osteoporosis patients in oral rehabilitation procedures.
OBJECTIVES:Alendronate and raloxifene, a bisphosphonate and a selective estrogen modulator, respectively, are established osteoporosis therapies. Current evidence suggests that simultaneous application of osteoporosis therapies modulates osseointegration. However, alendronate shows inconsistent findings and raloxifene has not been studied comprehensively. This study aimed to evaluate the bone dynamics and molecular and microstructural features at the peri-implant bone interface in osteoporoticrats. MATERIALS AND METHODS: Thirty female rats underwent ovariectomy and were fed a diet low in calcium and phosphate and treated with alendronate or raloxifene for 30 days or underwent fictional ovariectomy surgery (SHAM) prior to implant insertion in the tibia; osteoporosis therapies continued thereafter. After 42 days, peri-implant bone was evaluated by histometric and micro-CT analysis. Fluorochrome incorporation and gene expression was determined to evaluate bone turnover. RESULTS: We report here that alendronate had no impact on bone-to-implant contacts and the mineral apposition rate. The RANKL/OPG ratio and local bone volume, however, were increased compared to the untreated osteoporoticrats. Even though signaling to bone resorption activity through RANKL production was observed in the alendronate group, the blockade of bone resorption activity that occurs in decorrence to alendronate activity took place and resulted in an increase in bone volume. Raloxifene significantly increased osseointegration in osteoporoticrats, as indicated by bone-to-implant contacts, mineral apposition, and local bone volume. Raloxifene, however, had no considerable impact on the RANKL/OPG ratio compared to untreated osteoporoticrats. As expected, the SH group showed higher bone-to-implant contacts and mineral apposition rates than the untreated osteoporoticrats. CONCLUSIONS: These findings suggest that raloxifene but not alendronate can compensate for the impaired osseointegration in osteoporoticrats. CLINICAL RELEVANCE: Regarding the superiority of raloxifene observed in the improvement of bone dynamics response, this statement suggests that raloxifene could be a good option for osteoporosispatients in oral rehabilitation procedures.
Authors: Marcio A de Oliveira; Denise A Asahi; Celey A E Silveira; Luiz Antonio P A Lima; Michael Glick; Marina Gallottini Journal: Clin Oral Implants Res Date: 2014-01-20 Impact factor: 5.977
Authors: Ana Paula Farnezi Bassi; Vinícius Ferreira Bizelli; Leticia Freitas de Mendes Brasil; Járede Carvalho Pereira; Hesham Mohammed Al-Sharani; Gustavo Antonio Correa Momesso; Leonardo P Faverani; Flavia de Almeida Lucas Journal: Membranes (Basel) Date: 2020-09-12